| First Author | Honey NK | Year | 1984 |
| Journal | Cytogenet Cell Genet | Volume | 37 (1-4) |
| Pages | 492-493 (Abstr.) (399-616) | Mgi Jnum | J:4779 |
| Mgi Id | MGI:53261 | Citation | Honey NK, et al. (1984) Assignment in mouse of the genes for chymotrypsinogen B, elastase, trypsin, and carboxypeptidase A (Abstracts of meeting presentations: Human gene mapping 7, Los Angeles Conference (1983) Seventh International Workshop on Human Gene Mapping). Cytogenet Cell Genet 37 (1-4):492-493 (Abstr.) (399-616) |
| abstractText | Full text of Abstract: Assignment in mouse of the genes for chymotrypsinogen B, elastase, trypsin, and carboxypeptidase A. N.K. Honey,1 A.Y. Sakaguchi,1 P.A. Lalley,2 C. Quinto,3 R.J. MacDonald,4 W.J. Rutter,5 and S.L. Naylor1; lRoswell Park Memorial Institute, Buffalo, NY, 2Oak Ridge National Laboratory, Oak Ridge, TN, 3Centro de Figacion de Nitrogeno, Cuernavaca, Mexico, 4U. of Texas Health Science Center at Dallas, TX, and 5U. of California at San Francisco. CA. The genes for the serine endopeptidases chymotrypsinogen B (Ctrb) elastase-1 (Ela-1), and trypsin-1 (Trp-1), and the exopeptidase carboxypeptidase A (Cpa), have been isolated from a rat cDNA library. In order to map these genes in mouse, DNA from mouse x Chinese hamster hybrids was cleaved with Hind III or Bam HI and hybridized to Ctrb, Ela-1, Trp-1, or Cpa cDNA probes. Mouse gene sequences, detected on 5.3 kb and 3.1 kb fragments, segregated only with chromosome 8. Mouse Ela-1 gene sequences, detected on 10 kb and 4.2 kb fragments, segregated only with chromosome 15. Trp-1 gene sequences, detected on 12.8 kb, 9.2 kb, and 6.5 kb fragments, and Cpa gene sequences, detected on 5.2 kb and 3.7 kb fragments, both segregated only with chromosome 6. These data identify several conserved linkage groups. The human homolog of Ctrb maps to human chromosome HSA 16 and therefore comprises part of the region of homology previously observed between mouse chromosome (MMU) 8 and HSA 16. We have mapped the human homologs of both Trp-1 and Cpa to HSA 7q22->qter, and thus a region of homology exists between MMU6 and HSA7q. |
