| First Author | Hamvas RMJ | Year | 1990 |
| Journal | Mouse Genome | Volume | 87 |
| Pages | 114 | Mgi Jnum | J:23688 |
| Mgi Id | MGI:71559 | Citation | Hamvas RMJ, et al. (1990) The genetic mapping of an EAG I linking clone, EM131, to the ZFX/AR cluster on the mouse X Chromosome. Mouse Genome 87:114 |
| abstractText | Full text of Mouse Genome contribution: THE GENETIC MAPPING OF AN EAG I LINKING CLONE, EM 131, TO THE ZFX/AR CLUSTER ON THE MOUSE X CHROMOSOME. R.M.J. Hamvas, N. Brockdorff(1), JT. Keer and S.D.M. Brown; Dept. of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, London W2 lPG, UK; (1)Section of Comparative Biology, MRC Clinical Research Centre, Harrow, Middlesex HA1 3UJ, UK. The mouse X-inactivation centre is located just distal to the T16H breakpoint on the mouse X chromosome. Interspecific backcrosses have been used to map probes distal to the T16H breakpoint and in the vicinity of the Ta locus in order to generate a detailed genetic map in the region of the X-inactivation centre (1). EM131 is a new mouse genomic clone derived from a CpG-rich island library constructed from a somatic cell hybrid line, C1.8, which contains the entire X as the only mouse chromosome. A number of linking clones from this library, including the Eag I linking clone EM131, were initially localised distal to the T16H breakpoint and proximal to the T14R1 breakpoint (2). We now report the detailed genetic mapping of EM131 within this region. Two Mus spretus/Mus domesticus interspecific crosses were utilised to define the genetic map position of EM131: one in which backcross progeny segregate for the rndx mutation and the other in which probes are mapped with respect to the coat-texture mutations, Hq and Ta (see Ref. 1). A 1.3 kb Eco Rl subclone of Em131 hybridised to Taq I digested Mus spretus and Mus domesticus DNA identified single bands of 6.8 kb and 2.5 kb respectively (Fig 1 A, B). In addition, thirteen mice with recombination breakpoints in the vicinity of the Ta locus and distal to the T16H breakpoint were analysed with EM131. Pedigree analysis indicated that the probe was inseparable from the Zfx/Ar cluster and provides a new marker in the vicinity of the X-inactivation centre. 1. Keer, J.T., Hamvas, R.M.J., Brockdorff, N., Page, D., Rastan, S. and Brown, S.D.M. (1990) Genomics In press. 2. Brockdorff, N., Montague, M., Smith, S. and Rastan, S. (1990) Genomics In press. Fig. 1. (Legend). Pedigree analysis of EM131 using recombinant backcross progeny. 13 of 16 progeny demonstrating recombination events in the Dmd - Pgk-1 interval were analysed for the domesticus 2.5kb (A) and spretus 6.8kb (B) restriction fragment length variant. Brackets indicate loci that are physically and genetically inseparable. The approximate chromosomal location of Dmd, Zfx and Pgk-1 as identified by in situ hybridisation is indicated. |
