| First Author | Vincek V | Year | 1993 |
| Journal | Mouse Genome | Volume | 91 |
| Issue | 2 | Pages | 337-38 |
| Mgi Jnum | J:12811 | Mgi Id | MGI:61029 |
| Citation | Vincek V, et al. (1993) Genetic mapping of the mouse t complex distal inversion. Mouse Genome 91(2):337-38 |
| abstractText | Full text of Mouse Genome contribution: GENETIC MAPPING OF THE MOUSE t COMPLEX DISTAL INVERSION. Vladimir Vincek*, Howard C. Passmore(o), Zdenek Trachtulec*|, Felipe Figueroa+, and Jan Klein*+. * Department of Microbiology, University of Miami School of Medicine, Miami, FL 33136, USA. o Department of Biological Sciences, Rutgers University, Piscataway, NJ, 08855, USA. | Laboratory of Mammalian Molecular Genetics, Czech Academy of Sciences, 14220 Prague, Czech Republic. + Max-Planck Institut fur Biologie, Abteilung Immunogenetik, 7400 Tubingen, Germany. INTRODUCTION The linkage disequilibrium of genetic markers within the mouse t complex is caused by four inversions that suppress crossing-over (1). While the limits of the proximal inversions are fairly well determined, those of the distal inversion are much less so. Since we have obtained several new DNA markers (D17Tu) which in wild-type chromosome map between the mouse H-2 complex and the end of the distal inversion of the mouse chromosome 17, we have initiated a study to determine the position of our DNA markers relative to previously isolated probes. MATERIALS AND METHODS High molecular mass chromosomal DN prepared from a panel of mouse strains listed in Table 1 and described by Passmore and Romano (2). Genomic DNA (10 ug per strain) was digested with the appropriate restriction enzyme, electrophoresed on a 0.8% agarose gel, and transferred to a nylon membrane. Oligolabelling of DNA probes, hybridization, and washing were carried out as described elsewhere (3). The D17Tu probes were described by Vincek and coworkers (3). The D17Leh probes were obtained from Dr. H. Lehrach, Imperial Cancer Research Fund Laboratories, The Mb-1 probe was a gift from Dr. S. Singer, NIH, National Cancer Institute, Bethesda, MD, USA. The restriction enzymes used to detect restriction fragment length polymorphism (RFLP) and the size of variable fragments were as described earlier (3). RESULTS In this report we have characterized fifteen intra-Mhc recombinant mouse strains for the restriction fragment length polymorphiim of ten anonymous DNA probes localized to the distal end of the mouse t complex. The resulting strain distribution patterns (SDP) of individual loci were arranged in a tabular form together with SDPs of previously tested loci (Table 1). Based on these results we were able to divide all tested D17Tu probes previously mapped to the same cluster, into three clusters and to determine their position relative to the D17Leh markers (Table 1). However, the relative positions of the D17Tu and the D17Leh DNA markers within the individual clusters is still unknown. Table 1. Strain: MA.R1; Locus: H-2D: D; 50.21: D; H-2L: D; 46.21: D; Qa-2: D; 36.21: D; Tla: D; 22.11: D; 12.21:D; GRC: D; T1: D; D17Leh108*: D; D17Tu19*: D; Mb1*: D; D17Leh171*: D; D17Tu32*: D; D17Tu42*: D;| D17Tu49*: M; D17Leh525*: M; D17Leh467*: M; Tpx-1: M; D17Tu26*: M; Mut: M; Ce-2: M. Strain: MA.R2; Locus: H-2D: D; 50.21: D; H-2L: D; 46.21: D; Qa-2: D; 36.21: D; Tla: D; 22.11: D; 12.21:D; GRC: D; T1: D; D17Leh108*: D; D17Tu19*: D; Mb1*: D; D17Leh171*: D; D17Tu32*: D; D17Tu42*: D; D17Tu49*: D; D17Leh525*: D; D17Leh467*: D; Tpx-1: D; D17Tu26*: D; Mut: D;| Ce-2: M. Strain: MA.R3; Locus: H-2D: D; 50.21: D; H-2L: D;| 46.21: M; Qa-2: M; 36.21: M; Tla: M; 22.11: M; 12.21: M; GRC: M; T1: M; D17Leh108*: M; D17Tu19*: M; Mb1*: M; D17Leh171*: M; D17Tu32*: M; D17Tu42*: M; D17Tu49*: M; D17Leh525*: M; D17Leh467*: M; Tpx-1: M; D17Tu26*: M; Mut: M; Ce-2: M. Strain: MA.R4; Locus: H-2D: D; 50.21: D; H-2L: D; 46.21: D; Qa-2: D; 36.21: D;| Tla: M; 22.11: M; 12.21: M; GRC: M; T1: M; D17Leh108*: M; D17Tu19*: M; Mb1*: M; D17Leh171*: M; D17Tu32*: M; D17Tu42*: M; D17Tu49*: M; D17Leh525*: M; D17Leh467*: M; Tpx-1: M; D17Tu26*: M; Mut: M; Ce-2: M. Strain: MA.R5; Locus: H-2D: M; 50.21: M; H-2L: M; 46.21: M; Qa-2: M; 36.21: M; Tla: M; 22.11: M; 12.21: M; GRC: M; T1: M; D17Leh108*: M; D17Tu19*: M; Mb1*: M; D17Leh171*: M; D17Tu32*: M; D17Tu42*: M; D17Tu49*: M; D17Leh525*: M; D17Leh467*: M;| Tpx-1: D; D17Tu26*: D; Mut: D; Ce-2: D. Strain: B10.DR1; Locus: H-2D: D; 50.21: D; H-2L: D; 46.21: D; Qa-2: D; 36.21: D; Tla: D; 22.11: D; 12.21: D; GRC: D; T1: D; D17Leh108*: D; D17Tu19*: D; Mb1*: D; D17Leh171*: D; D17Tu32*: D; D17Tu42*: D; D17Tu49*: D; D17Leh525*: D; D17Leh467*: D;| Tpx-1: K; D17Tu26*: K; Mut: K; Ce-2: K. Strain: B10.DR2; Locus: H-2D: D; 50.21: D; H-2L: D; 46.21: D; Qa-2: D; 36.21: D;| Tla: K; 22.11: K; 12.21: K; GRC: K; T1: K; D17Leh108*: K; D17Tu19*: K; Mb1*: K; D17Leh171*: K; D17Tu32*: K; D17Tu42*: K; D17Tu49*: K; D17Leh525*: K; D17Leh467*: K; Tpx-1: K; D17Tu26*: K; Mut: K; Ce-2: K. Strain: B10.DR3; Locus: H-2D: D; 50.21: D; H-2L: D; 46.21: D; Qa-2: D; 36.21: D;| Tla: K; 22.11: K; 12.21: K; GRC: K; T1: K; D17Leh108*: K; D17Tu19*: K; Mb1*: K; D17Leh171*: K; D17Tu32*: K; D17Tu42*: K; D17Tu49*: K; D17Leh525*: K; D17Leh467*: K; Tpx-1: K; D17Tu26*: K; Mut: K; Ce-2: K. Strain: B10.KR1; Locus: H-2D: K; 50.21: K; H-2L: K; 46.21: K; Qa-2: K; 36.21: K;| Tla: D; 22.11: D; 12.21: D; GRC: D; T1: D; D17Leh108*: D; D17Tu19*: D; Mb1*: D; D17Leh171*: D; D17Tu32*: D; D17Tu42*: D; D17Tu49*: D; D17Leh525*: D; D17Leh467*: D; Tpx-1: D; D17Tu26*: D; Mut: D; Ce-2: D. Strain: B10.KR2; Locus: H-2D: K; 50.21: K; H-2L: K; 46.21: K; Qa-2: K; 36.21: K; Tla: K; 22.11: K; 12.21: K; GRC: K; T1: K; D17Leh108*: K; D17Tu19*: K; Mb1*: K; D17Leh171*: K; D17Tu32*: K; D17Tu42*: K; D17Tu49*: K; D17Leh525*: K; D17Leh467*: K; Tpx-1: K; D17Tu26*: K; Mut: K;| Ce-2: D. Strain: B10.KR3; Locus: H-2D: K; 50.21: K; H-2L: K; 46.21: K; Qa-2: K; 36.21: K;| Tla: D; 22.11: D; 12.21: D; GRC: D; T1: D; D17Leh108*: D; D17Tu19*: D; Mb1*: D; D17Leh171*: D; D17Tu32*: D; D17Tu42*: D; D17Tu49*: D; D17Leh525*: D; D17Leh467*: D; Tpx-1: D; D17Tu26*: D; Mut: D; Ce-2: D. Strain: B10.MR1; Locus: H-2D: M; 50.21: M; H-2L: M; 46.21: M; Qa-2: M; 36.21: M;| Tla: D; 22.11: D; 12.21: D; GRC: D; T1: D; D17Leh108*: D; D17Tu19*: D; Mb1*: D; D17Leh171*: D; D17Tu32*: D; D17Tu42*: D; D17Tu49*: D; D17Leh525*: D; D17Leh467*: D; Tpx-1: D; D17Tu26*: D; Mut: D; Ce-2: D. Strain: B10.MR2; Locus: H-2D: M; 50.21: M; H-2L: M; 46.21: M; Qa-2: M; 36.21: M; Tla: M; 22.11: M; 12.21: M; GRC: M;| T1: D; D17Leh108*: D; D17Tu19*: D; Mb1*: D; D17Leh171*: D; D17Tu32*: D; D17Tu42*: D; D17Tu49*: D; D17Leh525*: D; D17Leh467*: D; Tpx-1: D; D17Tu26*: D; Mut: D; Ce-2: D. Strain: B10.MR3; Locus: H-2D: M; 50.21: M; H-2L: M; 46.21: M; Qa-2: M; 36.21: M; Tla: M; 22.11: M; 12.21: M; GRC: M; T1: M; D17Leh108*: M; D17Tu19*: M; Mb1*: M; D17Leh171*: M; D17Tu32*: M; D17Tu42*: M; D17Tu49*: M; D17Leh525*: M; D17Leh467*: M; Tpx-1: M; D17Tu26*: M; Mut: M;| Ce-2: D. Strain: B10.GR1; Locus: H-2D: K; 50.21: K; H-2L: K; 46.21: K; Qa-2: K; 36.21: K;| Tla: G; 22.11: G; 12.21: G; GRC: G; T1: G; D17Leh108*: G; D17Tu19*: G; Mb1*: G; D17Leh171*: G; D17Tu32*: G; D17Tu42*: G; D17Tu49*: G; D17Leh525*: G; D17Leh467*: G; Tpx-1: G; D17Tu26*: G; Mut: G; Ce-2: G. Table 1 (Legend). The origin of alleles is as follows: D, B10.D2; K, B10.K; M, MA/MyJ; G, B10.G. Data on H-2, 50.21, 46.21, Qa-2, 36.21, Tla, 22.11, 12.21, Tl, and Ce-2 are from Passmore and Romano (1987). Data on GRC are from Vincek et al (1980) and data on Tpx-1 are from Kasahara et al (1989). Data on Mut are from Sertic et al (1990). Vertical lines indicate crossover positions. Asterisks indicate probes tested in this study. 1. Committee for Mouse Chromosome 17 (1991). Mammalian Genome 1:5-29. 2. Passmore, H.C. and Romano, J. (1987). In: H-2 Antigens, Genes, Molecules, Function. (C.S. David, ed.), pp. 49-60, Plenum Press, New York. 3. Vincek, V., Kawaguchi, H., Mizuno, K., Zaleska-Rutczynska, Z., Kasahara, M., Forejt, J., Figueroa, F., and Klein, J. (1989). Genomics 5:773-786. 4. Vincek, V., Figueroa, F., Gill, T.J., Cortese Hassett, A.L., and Klein, J. (1990) Immunogenetics 332:293-295. 5. Kasahara, M., Passmore, H.C., and Klein, J. (1989). Immunogenetics 29:61-63. 6. Sertic, J., Vincek, V., Ledley, F.D., Figueroa, F., and Klein, J. (1990). Genomics 6:560-564. |
