| First Author | Cumming RB | Year | 1978 |
| Journal | Mouse News Lett | Volume | 59 |
| Pages | 47 | Mgi Jnum | J:13781 |
| Mgi Id | MGI:61960 | Citation | Cumming RB, et al. (1978) Multiple forms of formamidase in the mouse. Mouse News Lett 59:47 |
| abstractText | Full text of MNL contribution: B. Multiple forms of formamidase in the mouse. We previously reported the presence of two biochemically and genetically distinct forms of formamidase (E.C. 3.5.1.9) in mouse liver (Mouse News Letter 56: 53, 1977). These were designated formamidase-1 and formamidase-2. Most mouse tissues have detectable formamidase activi ty and in some cases the enzymes involved are different from those in the liver. For the kidney, two peaks of formamidase activity can be resolved by DEAE cellulose chromatography. The second of these is the same as fomamidase-1 of the 1iver. The first peak to elute from DEAE columns for kidney homogenates (co-chromatographs with formamidase-2 of the liver) is actually composed of two different enzymes. These two enzyme activities can be separated from each other by ammonium sulfate fractionation. We have called these two enzymes formamidase-3 and formamidase-4. There are allelic forms of these enzymes in various inbred strains of mice and these forms can be distinguished from each other by heat stability and substrate affinity. There exists a formamidase in the brain of the mouse which is biochemically and genetically distinct from all of the liver and kidney formamidases. We have called this enzyme formamidase-5 and designated the structural gene for it For-5. Two alleles, For-5b and For-5d, have been described and their distribution has been determined in 46 inbred strains and substrains of mice. The two alleles can be distinguished by their heat stability. The For-5 locus has been mapped to Chromosome 14 and is very closely linked to Es-10 and hr (Cumming et a1., Biochem. Genet., in press). With the exception of formamidase-1, the physiological significance of these enzymes is not known. Studies are in progress on their biochemistry and genetics. (R. B. Cumming, M. F. Walton and F. H. Gaertner). |
