| First Author | Edelhoff S | Year | 1992 |
| Journal | Am J Hum Genet | Volume | 51 |
| Issue | 4 | Pages | A395 (Abstr.) |
| Mgi Jnum | J:2928 | Mgi Id | MGI:51445 |
| Citation | Edelhoff S, et al. (1992) Mapping of the glutamic acid decarboxylase (GAD) gene family. Am J Hum Genet 51(4):A395 (Abstr.) |
| abstractText | Full text of Abstract: Physical Mapping (continued). Mapping of the glutamic acid decarboxylase (GAD) gene family. S. Edelhoff1, C.E. Grubin2, A.E. Karlsen2, D.A. Adler1, A. Lernmark2, C.M. Disteche1. Depts. Of Pathology1 and Medicine2, University of Washington, Seattle, WA. Glutamic acid decarboxylase (GAD) catalyzes the conversion of glutamic acid to gamma-aminobutyric acid (GABA). GABA is known as the major inhibitory neurotransmitter in the central nervous system (CNS) of vertebrates, but is also present outside the CNS. Recent studies showed GAD as a major target of autoantibodies associated with the development of insulin-dependent diabetes mellitus. Studies of GAD expression demonstrated many different isoforms of the gene. Three different isoforms, GAD1, GAD2, and GAD3, which form a family of highly conserved genes, have been isolated. We mapped the GAD gene family by in situ hybridization both in human and in mouse. In situ hybridization to human and mouse metaphase chromosomes was carried out using highly stringent conditions to avoid cross hybridization between the genes. We mapped GAD1 to human chromosome 2q31 and to mouse chromosome 2D in a known region of conservation between human and mouse. GAD2 was mapped to chromosome 10p11.2-p12 in human and to chromosome 2A2-B in mouse. This defines a new region of conservation between human and mouse chromosomes. GAD3 was mapped to human chromosome 22q13 and to mouse chromosome 15E in a known region of conservation between human and mouse. |
