| First Author | Knight AM | Year | 1992 |
| Journal | Mouse Genome | Volume | 90 |
| Issue | 2 | Pages | 222 |
| Mgi Jnum | J:1272 | Mgi Id | MGI:49802 |
| Citation | Knight AM, et al. (1992) Novel RFLP using H2b histone 3' flanking sequence maps to mouse chromosome 11. Mouse Genome 90(2):222 |
| abstractText | Full text of Mouse Genome contribution: NOVEL RFLP USING H2b HISTONK 3' FLANKING SEQUENCE MAPS TO MOUSE CHROMOSOME 11. Andrew M. Knight1, William F. Marzluff2 and P. Julian Dyson1, Section of Transplantation Biology, Clinical Research Centre, Watford Road, Harrow, Middx. HA1 3UJ, U.K.1 and The University of North Carolina at Chapel Hill, 101 Molecular Biology & Biotechnology Research Laboratories, Campus Box 7100, Chapel Hill, North Carolina, 27599-7100, USA.2 Mouse histone genes are encoded from two complexes, Hist1 and Hist2 located on chromosomes 13 and 3 respectively [1, 2]. In this report we show that a probe containing the 3' flanking region of the H2b gene [3] from the Hist1 complex detects two independently segregating restriction-length polymorphisms (RFLPs). Subsequent analysis, using the BXD series of recombinant inbred strains [4] reveals that one of these RFLPs maps to the Hist1 locus on chromosome 13 and the other maps to a novel location on chromosome 11. Materials and Methods Genomic liver DNA was prepared from C57BL/6CRC and DBA/2CRC mice as described [5]. DNA from the BXD series was purchased from the Jackson Laboratory, Bar Harbor, Maine, U.S.A. After overnight digestion at 37 degrees C with Bgl II, according to manufacturer's conditions (New England Biolabs U.K.), DNAs were electrophoresed through 0.7% agarose and transferred to nylon filters (Genescreen plus II, Dupont). Hybridization, with an 800bp Sst I insert from the plasmid MH143 [3] containing the 3' flanking region of the H2b gene, labelled using random oligonucleotide primer extension in the presence of 32P dCTP, was carried out overnight at 65 degrees C and filters subsequently washed at high stringency (0.1 XSSC, 0.1% SDS) for 2 hours at 63 degrees C. Description of polymorphisms and segregation analysis The probe hybridized to a number of bands. However, a dominant band of approximately 8 Kb was seen to be present in DBA/2 with a 9 Kb dominant hand being present in C57BL/6. This polymorphism segregated with the Hist1 locus on chromosome 13 (data not shown). An additional band of approximately 16 Kb was present only in C57BL/6. A corresponding DBA/2 band was not seen, this may be due to it being either obscured by other bands or absent from DBA/2. The 16Kb band was used to re-type the BXD series; its segregation was independent of the other polymorphism, and co-segregated with markers on chromosome 11 with 1/25 mismatches with the SDP of IL-5 [6] indicating a distance of 1.06 cM. (95% confidence interval of 0.03-7.32 cM; [7]). Locus: Hist3; 1: D; 2: D; 5: B; 6: D; 8: B; 9: B; 11: B; 12: D; 13: D; 14: D; 15: B; 16: D; 18: B; 19: D; 20: B; 21: D; 22: B; 23: B; 24: B; 25: B; 27: B; 28: D; 29: D; 30: B; 31: D; 32: U. Locus: Il-5; 1: D; 2: D; 5: B; 6: D; 8: B; 9: B; 11: B; 12: D; 13: D; 14: D; 15: B; 16: D; 18: B; 19: B; 20: B; 21: D; 22: B; 23: B; 24: B; 25: B; 27: B; 28: D; 29: D; 30: B; 31: D; 32: D. Reference [6]. Comments The probe MH143 contains DNA from the 3' flanking region of the H2b histone gene located on chromosome 13 in the Hist1 complex [3]. We have shown, using a RFLP detected by this probe, that this sequence is also present on chromosome 11. The simplest explanation for this new location is that a duplication and subsequent translocation event may have led to related sequences being present on chromosome 11. References 1. D.R. Cox et a1 (1984) Cytogenet. Cell Genet. 37:443 (Abstr.) 2. R.A. Graves et al (1985) J. Mol. Biol. 183: 179-194. 3. Y-S. Yang et al (1987) J. Biol. Chem. 262: 17118-17125. 4. B.A. Taylor. (1989) In Lyon, M.F. and Searle, A.G. (eds.). Genetic Variants and Strains of the Laboratory Mouse. University Press, Oxford. second ed., pp. 773-796. 5. T. Maniatas et al (1982) In Molecular Cloning: A laboratory manual (Cold Spring Harbor Laboratory, New York). 6. Wilson et a1 (1989) Mouse News Lett. 85: 14-16. 7. J. Silver (1985) The Journal of Heredity 76: 436-440. |
