| First Author | Miller RD | Year | 1993 |
| Journal | Mouse Genome | Volume | 91 |
| Issue | 1 | Pages | 133 |
| Mgi Jnum | J:4261 | Mgi Id | MGI:52757 |
| Citation | Miller RD, et al. (1993) Orientation of mahoganoid relative to Prm-2. Mouse Genome 91(1):133 |
| abstractText | Full text of Mouse Genome contribution: ORIENTATION OF MAHOGANOID RELATIVE TO Prm-2. R. D. Miller and R. Riblet; Medical Biology Institute, La Jolla, California, 92037, USA. mohoganoid (md), a recessive coat color mutation, arose spontaneously in the C3H/HeJ strain (1). Agouti mice homozygous for md show a darkening of the dorsal hairs, ears and tail. Of historical interest, md was the first marker assigned to Chr. 16 by linkage to the Robertsonian translocation Rb(16.17)Bnr; and with this assignment all mouse chromosomes had at least one associated gene (2). We have oriented md relative to Chr. 16 reference loci (3) by typing 70 progeny of the intraspecific backcross of female (C3H/HeJ-md x SJL)Fl x male C3H/HeJ-md for the md phenotype and the Prm-2 (protamine-2) and Igl-I (immunoglobulin gamma light chain) reference loci. The simple repeat sequence markers D16Mit1, D16Mit2, D16Mit8, D16Mit9, D16Mit29, and D16Mit31 which map to the centromeric region of Chr. 16 were not polymorphic in this cross. Prm-2 was typed by Southern blot analysis of EcoRI digested genomic DNA hybridized with the pmP-2 probe provided by Dr. Roger Reeves (Johns Hopkins University). This probe is a cDNA clone which hybridizes to a 16 kb EcoRI fragment in C3H/HeJ-md DNA and a 7 kb EcoRI fragment in SJL DNA. As reported by Reeves and colleagues (4) for other mouse strains, Prm-2 restriction fragment length variants were also detected between C3H/HeJ-md and SJL with EcoR V, BstE II, Bgl II, and Xba I. Igl-1 was typed by Southern blot analysis of Kpn I digested genomic DNA hybridized with the pCgamma1 4 E probe provided by Dr. Ruth Epstein (Medical Biology Institute). This probe contains the Cgamma1 constant region and hybridizes to a 7 kb Kpn I fragment in C3H/HeJ-md DNA and an 11 kb Kpn I fragment in SJL DNA. Hybridization conditions were as described previously (5). A summary of the typing is presented in Figure 1. These results help to establish the relative gene order and linkage distances shown in Figure 2. This linkage map is consistent with the orientation of Igl-1 distal to Prm-2 (3) and the work of Epstein and colleagues (6) which established the orientation of Igl-1 distal to md. Figure 1. (Legend). Frequency and haplotype of maternal (F1) chromosome in 70 backcross progeny. Figure 2. (Legend). Linkage map of MMU16 over the interval containing the md mutation. Distances between loci are expressed in cM +/- standard error determined using the backcross statistics function of the RI Manager program version 2.2 (7). References: 1. Lane, Mouse News Letter 22:35, 1960. 2. Roderick et al., Mouse News Letter 55:18, 1976. 3. Reeves and Miller, Mammalian Genome 3:S233. 1992. 4, Reeves et al. J. Hered. 80:442, 1989. 5. Miller et al., Immunogenetics 30:511, 1989. 6. Epstein et al. Immunogenetics 23:78, 1986. 7. Manly and Elliott, Mammalian Genome 1:123, 1991. |
