| First Author | Kinzler KW | Year | 1987 |
| Journal | Cytogenet Cell Genet | Volume | 46 |
| Pages | 639 (Abstr.) | Mgi Jnum | J:12422 |
| Mgi Id | MGI:60669 | Citation | Kinzler KW, et al. (1987) Identification of an amplified, highly expressed gene in a human glioma. Cytogenet Cell Genet 46:639 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of workshop presentations. Identification of an amplified, highly expressed gene in a human glioma. Kinzler KW1, Bigner SH2, Bigner DD2, Trent JM3, Law ML4, O'Brien SJ5, Wong AJ1, Vogelstein B1. 1Johns Hopkins University School of Medicine, Baltimore, MD. 2Duke University Medical Center, Dunham, NC. 3University of Arizona, Tucson, AZ. 4University of Colorado, Denver, CO. 5Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, MD. A cell line, D-259 MG, derived from a malignant glioma from a 46 year old male contained numerous double chromosomes suggesting gene amplification. Molecular evidence for gene amplification of this tumor line was obtained with the denaturation-venaturation gel technique developed by I. Roninson. A plasmid library enriched for amplified sequences was developed to isolate the normally single copy sequences, termed GLI, which were amplified more than 50 fold in the D259 MG cell line. The GLI gene Was assigned to human chromosome 12 by Southern analysis of DNA from a panel of rodent x human somatic cell hybrids. By similar analysis using hybrids with portions of human chromosome 12 and by in situ hybridization to metaphase chromosomes, we regionally localized GLI to 12q13-14.3. The GLI gene is transcriptionally active in the parent cell line and in other tissues. The GLI gene is a member of a select group of cellular genes that are genetically altered in primary human tumors. HGM symbol: GLI |
