| First Author | Yang-Feng TL | Year | 1987 |
| Journal | Cytogenet Cell Genet | Volume | 46 |
| Pages | 723 (Abstr.) | Mgi Jnum | J:12307 |
| Mgi Id | MGI:60555 | Citation | Yang-Feng TL, et al. (1987) Gene for the human signal recognition particle receptor (SRPR) mapped to Chromosome 11q24-q25. Cytogenet Cell Genet 46:723 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of workshop presentations. Gene for the human signal recognition particle receptor (SRPR) mapped to chromosome 11q24-q25. Yang-Feng TL,l Lauffer L,2 Walter P,2 Francke U.1 1Department of Human Genetics, Yale University School of Medicine, New Haven, CT. 2Department of Biocheniistry and Biophysics, University of California, San Francisco, CA. The signal recognition particle receptor (SRPR), also termed "docking protein", is an integral membrane protein of the endoplasmic reticulum (ER). Interaction of the SRPR with the signal recognition particle (SRP) on the arrested ribosome is essential for the protein targeting process in the ER membrane. SRP recognizes the signal sequences of nascent secretary proteins and arrests the elongation of the polypeptide chain thus preventing completion of the presecretory protein. The SRPR releases the arrest and targets the nascent polypeptide chain to the membrane. We have used both 5' (SR1) and 3' (SR50) SRPR cDNA clones, isolated from a dog kidney cell line cDNA library (Lauffer et al. Nature 318:314,1985) to map the SRPR gene in the human. Of 140 cells analyzed after in situ chromosomal hybridization with 3H-labeled SR1, 23 (16.4%) had silver grains at bands q24-q25 of chromosome 11. Of 251 grains scored, 23 (9.2%) were found over this region. DNA from 16 human x Chinese hamster hybrids and one human x mouse hybrid was digested with HindIII and hybridized to SRPR cDNA sequences. One human-specific fragment (5.8kb) was present in all the hybrids containing human chromosome 11. Hybridization was also positive in two subclones of a hybrid carrying only the long arm of human chromosome 11 but was negative in one hybrid containing only 11p. Two Chinese hamster specific fragments (3kb and 1.4kb) were detected by probe SR1 but only the 1.4kb fragment hybridized with probe SR50. These data from Southern blot analysis confirmed the localization of SRPR to chromosome 11, bands q24-q25, made by in situ hybridization. HGM symbol : SRPR |
