| First Author | Handel MA | Year | 1992 |
| Journal | Isozyme Bulletin | Volume | 25 |
| Pages | 36 (Abstr.) | Mgi Jnum | J:1367 |
| Mgi Id | MGI:49894 | Citation | Handel MA, et al. (1992) Genetic approaches to analysis of LDH-C expression during spermatogenesis in the mouse. Isozyme Bulletin 25:36 (Abstr.) |
| abstractText | Full text of Abstract: Isozyme Bulletin 25; 1992. GENETIC APPROACHES TO ANALYSIS OF LDH-C EXPRESSION DURING SPERMATOGENESIS IN THE MOUSE. Mary Ann Handel,* Erwin Goldberg,+ Wentong Zhou,+ Lynn Lameier,* Eugene M. Rinchik.# *University of Tennessee, Knoxville, TN, +Northwestern University, Evanston, IL, #Oak Ridge National Laboratory, Oak Ridge, TN. We have accomplished regional mapping of the mouse Ldh-3 gene, encoding the C subunit of LDH, and have studied expression of the protein product in various male-sterile mouse mutants impaired in spermatogenesis. The first approach to mapping was deletion analysis. We found that Ldh-3 is deleted in only one (p 46DFiOD) of 23 tested p-locus lethal deletions; this deletion interval also includes the gene ru-2, which is proximal to p and distal to Ldh-1. Recombination analysis was performed by testing segregation in a Mus spretus backcross. No recombinations between Ldh-3 and Myod-1 or Ldh-1 were found in 184 segregants analyzed. Thus Myod-1, Ldh-1 and Ldh-3 segregate as a linked unit proximal to p on mouse Chromosome 7; this unit shows recombination with p in 9 of 184 mice analyzed. We have used indirect immunofluorescence with an antibody against the LDH-C subunit to study LDH-C4 expression in a number of different male-sterile mouse mutants. In all cases where spermatogenic cells progressing to early- or mid-pachytene were found, expression of LDH-C4 was also observed. Thus the various mutations studied do not interfere with the control of expression of LDH-C4. Furthermore, these results suggest that various aspects of spermatogenic-specific gene expression are independent, rather than mutually dependent, differentiative pathways. |
