| First Author | Floyd-Smith GA | Year | 1985 |
| Journal | Cytogenet Cell Genet | Volume | 40 |
| Pages | 630 (Abstr.) | Mgi Jnum | J:14876 |
| Mgi Id | MGI:63034 | Citation | Floyd-Smith GA, et al. (1985) The mouse pronatriodilatin gene (Pnd) is located on Chromosome 4. Cytogenet Cell Genet 40:630 (Abstr.) |
| abstractText | Full text of Abstract: The mouse pronatriodilatin gene (Pnd) is located on chromosome 4. C.A. Floyd-Smith,1 M. Nemer, 2 J. Drouin,2 and U. Francke1. 1Yale University, Department of Human Genetics, New Haven, CT, 2Clinical Research Institute of Montreal, Molecular Biology Laboratory, Montreal, Quebec Atrial natriuretic factors (ANF) are polypeptides ranging from 3,000 to 13,000d which are secreted from specialized cells in the atrium, and which act to increase the excretion of sodium from the kidney (deBold et al., Life Sciences 28;89, 1981) and to relax pre-contracted vascular smooth muscle (Currie et al., Science 221:71, 1983). Although multiple active forms of these polypeptides have been isolated, they appear to be encoded by a single gene, pronatriodilatin (Pnd). A Pnd cDNA of 600bp was isolated from a rat atrial cDNA library and cloned into the PstI site of pUC8 (Zivin et a1., Proc. Natl. Acad. Sci. USA 81:6325, 1984). This recombinant plasmid, pANF-44, was used as a probe to identify the chromosomal location of the murine gene using a panel of mouse x Chinese hamster somatic cell hybrids. Genomic DNA from mouse 3T3 fibroblasts, V79/380-6 Chinese hamster lung fibroblasts and 15 mouse x Chinese hamster somatic cell hybrids was digested to completion with EcoRI and the resulting fragments electrophoresed on agarose gels and transferred to nitrocellulose filters. Hybridization of pANF-44 to a 16kb fragment present in EcoRI digested Chinese hamster DNA and to a 6.7kb fragment in EcoRI digested mouse DNA was observed. The Pnd gene cosegregated with mouse chromosome 4 whereas all other chromosomes could be excluded by three or more discordant hybrids. Recently, the human PND gene was mapped to the distal short arm of chromosome 1 in band 1p36 (Yang-Feng et al., HGM 8). Assignment of the murine Pnd gene to chromosome 4 makes it part of the conserved syntenic group of homologous genes on mouse chromosome 4 and on the distal half of the short arm of human chromosome 1. |
