| First Author | Evans EP | Year | 1989 |
| Journal | Cytogenet Cell Genet | Volume | 51 |
| Pages | 995 (Abstr.) | Mgi Jnum | J:12529 |
| Mgi Id | MGI:60774 | Citation | Evans EP, et al. (1989) Localization of genes on the mouse X chromosome by in situ hybridization: the dystrophin locus maps to XC and the properdin locus to XA3. Cytogenet Cell Genet 51:995 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of workshop presentations: Localization of genes on the mouse X chromosome by in situ hybridization; the dystrophin locus maps to XC and the properdin locus to XA3. (A2616). EP Evans1,3, MD Burtenshaw1,3, D Goundis2, KBM Reid2, Y Boyd3. 1Sir William Dunn School of Pathology, University of Oxford, UK; 2MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, UK; 3Genetics Division, MRC Radiobiology Unit, Oxon, UK. The precise localization by in situ hybridization of homologous loci in mouse and man contributes to existing knowledge concerning the conservation and evolution of the mammalian X chromosome. We report here physical assignments on the mouse X chromosome for the dystrophin locus (DMD) and for the properdin locus two cloned genes whose location on the human X chromosome has previously been established (HGM9; Goundis & Reid, Nature 335:82-85, 1988). In each case probe DNA was labeled with 3H-dCTP and hybridized to metaphase chromosomes prepared from male mice or mice homozygous for the Rb(X2)2Ad Robertsonian translocation. When dystrophin was used as a probe, a total of 467 grains were scored over 108 complete metaphases and 54 of these were assigned to the X chromosome. A peak grain distribution (20 grains) was observed over the proximal section of band XC which is compatible with the location defined by genetic mapping studies using Mus domesticus/Mus spretus interspecific crosses. When the cDNA encoding mouse properdin was used as a probe, 116 grains were scored over 100 complete metaphases and 37 were assigned to the X chromosome. Further, two sites of hybridization were observed; a major site at the XA3 band (23/37 grains) and a second site at the junction of bands XD and XE (8/37 grains). The major site of hybridization is in the position predicted by the mapping of the homologous human locus to Xp11.23-Xp21.1 (Goundis et al., Genomics, in press). The nature of the second site of hybridization is as yet unclear. |
