| First Author | Ashley PL | Year | 1987 |
| Journal | Cytogenet Cell Genet | Volume | 46 |
| Pages | 573 (Abstr.) | Mgi Jnum | J:4870 |
| Mgi Id | MGI:53351 | Citation | Ashley PL, et al. (1987) An expressed phosphofructokinase gene (PFKX) maps to human Chromosome 12 and mouse Chromosome 15. Cytogenet Cell Genet 46:573 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of workshop presentations. An expressed phosphofructokinase gene (PFKX) maps to human chromosome 12 and mouse chromosome 15. Ashley PL, Price ER, Lebo RV, Cox DR. University of California, San Francisco, CA. Genetic loci coding for human phosphofructokinase (PFK) subunits have been mapped to human chromosome 21 (PFKL), 1 (PFKM) and 10 (PFKP). We report the use of a mouse PFK cDNA probe in conjunction with a human astrocytoma cDNA library to isolate a human PFK cDNA probe clone representing a fourth previously unrecognized human PFK gene (PFKX). DNA sequence analysis of the 2.0kb human PFKX cDNA insert and the homologous 2.6kb mouse cDNA insert reveals 86% and 84% nucleic acid sequence homology respectively when compared to the rabbit muscle PFK DNA sequence. We have assigned PFKX to human chromosome 12 using a single copy probe derived from the human PFKX cDNA in combination with spot blot hybridization of sorted human chromosomes and Southern blot hybridization of DNA from mouse x human somatic cell hybrids. In addition, we have assigned the homologous murine gene to mouse chromosome 15 using a panel of hamster x mouse somatic cell hybrids. SI nuclease protection assays indicate that PFKX is expressed in human fibroblasts as well as human brain. Northern blot analysis of RNA from adult mouse tissues reveals high levels of PFKX mRNA in mouse brain and testes. Previous studies have shown that three human chromosome 12 genes ELA1, INT1 and GPD1 are syntenic in the mouse and map to mouse chromosome 15. Our assignment of PFKX in man and mouse provides further evidence of homology between a segment of human chromosome 12 and mouse chromosome 15. HGM symbol: PFKX |
