| First Author | Harper ME | Year | 1984 |
| Journal | Cytogenet Cell Genet | Volume | 37 (1-4) |
| Pages | 488 (399-616) | Mgi Jnum | J:12542 |
| Mgi Id | MGI:60784 | Citation | Harper ME, et al. (1984) Gene mapping in the mouse by analysis of RFLP segregation in recombinant inbred strains. (Abstracts of meeting presentations: Human gene mapping 7, Los Angeles Conference (1983) Seventh International Workshop on Human Gene Mapping). Cytogenet Cell Genet 37 (1-4):488 (399-616) |
| abstractText | Full text of Abstract: Abstracts of meeting presentations. PAGE 488. Gene mapping in the mouse by analysis of RFLP segregation in recombinant inbred Strains. M.E. Harper,1 C. Blatt,1 S. Marks,1 M.N. Nesbitt,2 and M.I. Simon1. lThe Agouron Institute, La Jolla, CA, and 2Department of Biology, University of California, San Diego. A variety of single copy genes have been mapped in the mouse genome by segregation analysis of restriction fragment length polymorphisms (RFLPs) in recombinant inbred (RI) mice. In this approach, DNA probes corresponding to specific genes are used to screen for RFLP by Southern blot hybridization in parental strain DNAs [A/J (A), C57BL/6J (B) , DBA/2J (D)]. The hybridization pattern is then determined for each BxD or AxB RI line and the resulting strain distribution pattern (SDP) is compared with those of previously mapped genes for detection of linkage. Single copy genes mapped to date include alpha-fetoprotein to chromosome 5 (5 cM from Ric; 9 cM from Pgm-1); c-fes to chromosome 7 (<l cM from Gpi-1), c-src to chromosome 2 (2 cM from Psp), elastase-I to chromosome 4 (<l cM from Akp-2), and alphal-antitrypsin to chromosome 12 (9 cM from IgH-C). Probes corresponding to several dispersed low repeat gene families have also been used. These probes offer an additional advantage of rapid saturation of the genetic map since the likelihood of detecting RFLP is high. Mapping data on the Ul snRNA family includes one gene member on chromosome 1 (4 cM from Pep-3; 10 cM from Lsh), one member on chromosome 9 (<l cM from Sep-1), and one cluster of genes linked to Ace (5 cM) . This approach also allows the identification and characterization of chromosomal regions containing linkage relationships conserved between mouse and man. Based upon our mapping of the mouse elastase-I gene within a linkage group on chromosome 4 that is conserved between mouse and man, we predict that the human elastase-I gene is located on the short arm of chromosome 1. |
