| First Author | Saydak MM | Year | 1992 |
| Journal | FASEB J | Volume | 6 |
| Issue | 1 | Pages | A75 (Abstr.) |
| Mgi Jnum | J:681 | Mgi Id | MGI:49216 |
| Citation | Saydak MM, et al. (1992) Characterization of the mouse tissue transglutaminase gene. FASEB J 6(1):A75 (Abstr.) |
| abstractText | Full text of Abstract: MONDAY AM. STRUCTURE OF EUKARYTOTIC GENES AND GENOMES (429-433). CHARACTERIZATION OF THE MOUSE TISSUE TRANSGLUTAMlNASE GENE. Margaret M. Saydak, Joseph P. Stein and Peter J.A. Davies. Department of Pharmacology, University of Texas Medical School, Houston, TX 77225. Transglutaminases are a family of enzymes that catalyze the covalent cross-linking of proteins through the formation of e-(gamma-glutaminyl)-lysylisopeptide bonds. Tissue transglutaminase (Tgase) is an intracellular enzyme which is expressed in terminally differentiated and senescent cells and also in cells undergoing apoptotic cell death. Previous work in our laboratory has shown that the transcription of the Tgase gene is directly controlled by retinoic acid and retinoic acid receptors. To identify the regions of the Tgase gene responsible for regulating its expression, we have isolated and characterized a genomic clone encompassing the 5Õ end of the mouse Tgase gene. The transcription start site was defined using genomic and cDNA clones coupled with SI protection analysis and anchored PCR. This clone includes 2.3 kb upstream of the transcription start site and two exons that contain the first 256 nucleotides of the mouse Tgase cDNA sequence. The exon intron boundaries have been mapped and are different from the exon intron boundaries of human factor XIIIa subunit gene and human erythrocyte band 4.1 gene (two other members of the transglutaminase family). Preliminary analysis of the 5Õ flanking region of the Tgase gene indicates that it encodes a functional promoter with multiple potential retinoic acid regulatory elements. |
