| First Author | Blair HJ | Year | 1994 |
| Journal | Mouse Genome | Volume | 92 |
| Issue | 1 | Pages | 127-29 |
| Mgi Jnum | J:17345 | Mgi Id | MGI:65392 |
| Citation | Blair HJ, et al. (1994) Positioning of 14 simple sequence repeat loci relative to markers on the gene-based map of the mouse X Chromosome. Mouse Genome 92(1):127-29 |
| abstractText | Full text of Mouse Genome contribution: Positioning of 14 Simple Sequence Repeat Loci Relative to Markers on the Gene-based Map of the Mouse X Chromosome. Helen J. Blair and Yvonne Boyd; Genetics Division, MRC Radiobiology Unit, Chilton, Didcot, Oxon. OX11 ORD. The mouse X chromosome contains several loci involved in mouse mutant phenotypes which are potential models for human genetic disorders e.g. the mouse mutant tattered and the human disorder incontinentia pigmenti type 1 (IP1) (1). Only a few of these loci have been positioned with respect to molecular markers in intra- or interspecific backcrosses. Positioning of mutant loci on a high resolution genetic map (within 500-1000kb intervals) would greatly facilitate the isolation of genes involved in the phenotypes. Furthermore, mapping mutant loci with respect to evolutionary conserved sequences would allow their homology with human genetic diseases to be identified. Simple sequence repeats (SSR's, microsatellites) occur frequently in the mouse genome, are easily analysed using the polymerase chain reaction (PCR) and are often polymorphic between inbred mouse strains as well as between species (2,3). These sequences, although not conserved, are a useful way of integrating mutant phenotype maps with those created using conserved sequences and genes. We report here the position of 14 SSR loci relative to 23 conserved markers, including cloned genes, on the genetic map of the mouse X chromosome using two Mus musculus x Mus spretus backcrosses (4). A summary of the genetic distances and the order of markers determined is given in Figure la. Details of the mapping data derived from both backcrosses is presented in Figure 1b. All SSR markers were typed by PCR and analysed on 3% standard agarose gels (Sigma). Details of primer sequences, reaction conditions and product sizes for DXMit1 and DXMit3 were taken from Dietrich et at., 1992 (3). For all other SSRs, developed by Dietrich et al., this information was obtained from Research Genetics (Huntsville, Ala. USA). Mapping details of other loci used in this study are given in the following references: DXWas70 (5); DXF34-rs1, DXHX676, DXHX674 and DXHX679 (6); Tfe3 (7); Gata1 (8); Otc, Maoa and Pfc (9); Ndp (the murine homologue of the gene mutated in Norrie disease (Note: Ndph 8/5/95) pseudoglioma, Pagano et al., in preparation); Cdr, Gabra3 and Dmd (10); Xist, Plp, Pdha1 and Amg (4); Mnk and Pgk1 (11); Xlatk (xid,l2,13); Grpr (14); Glra2 (15). In conclusion, we have placed 14 SSR loci developed by Research Genetics on the genetic map of the mouse X chromosome using interspecific backcrosses and established their position with respect to many well-characterised loci. These markers span the length of the mouse X chromosome and will be valuable for mapping X-linked loci responsible for mouse mutant phenotypes. In addition, they provide useful tools for the isolation of YAC clones and in this way, integrated physical and genetic maps can be established. Figure 1. (Legend) (a) Genetic map of the mouse X chromosome established at the Genetics Division, MRC Radiobiology Unit. Loci shown in bold type have been used to type most offspring from both backcrosses. The genetic distances and recombination fractions between these loci are shown on the left of the map. Note: Some animals with recombination events in these intervals where not scored for both flanking loci therefore are not included in the genetic distance calculations, (b) Information obtained from both backcrosses by testing all animals with recombination events in selected intervals. The number of recombination events between each pair of loci is shown, with the total number of recombination events in the interval given below in brackets. The SSR loci are shown underlined. Mapping details of other loci are given in references in the text. Backcross 1 comprises 107 animals from a cross between (M. musculus (3H1) x M. spretus) females and M. musculus (3H1) males (9, l0). The laboratory stock, 3H1, is an F1 hybrid from a C3H/HeH female x 101/H male cross. Backcross 2 includes 84 animals from a similar backcross using the laboratory stock An, which is maintained on a C3H/HeH background, instead of 3H1 (4). Acknowledgments: This work was supported by the HGMP Directed Programme Committee. References (1) Davisson, M.T. (1987). Genomics 1:213-227. (2) Copeland, N.G. et al., (1993). Science 262: 57-66. (3) Dietrich, W. et al., (1992). Genetics 131: 423-447. (4) Blair, H.J. et al., (1993). Mamm. Genome 4: 230-233. (5) Disteche, C.M. et al., (1989). Genomics 5: 177-184. (6) Laval, S.H, and Boyd, Y. (1993) Genomics 15: 483-491. (7) Blair, H.J. and Boyd, Y. Mouse Genome: in preparation. (8) Laval, S.H. and Boyd, Y. (1993). Mamm, Genome 4: 119-123. (9) Laval, S.H. et al., (1991). Genomics 10: 1030-1034. (10) Laval, S.H. et al., (1992). Genomics 12: 818-821. (11) Reed, V, et al., (1994). Genetical Research, in press. (12) Hendricks, R. et al., (1994), Mamm. Genome 5 in press. (13) Thomas, J.D. et al., (1993). Science 261 : 355-358. (14) Maslen, G.L. and Boyd, Y. (1993). Genomics 17:106-109. (15) Derry, J. et al., (1991). Genomics 10:593-597. |
