| First Author | Kida H | Year | 1993 |
| Journal | J Assist Reprod Genet | Volume | Suppl 10 |
| Issue | 6 | Pages | 239 (Abstr) |
| Mgi Jnum | J:16147 | Mgi Id | MGI:64237 |
| Citation | Kida H, et al. (1993) Expression of tenascin in the mouse uterus during early pregnancy. J Assist Reprod Genet Suppl 10(6):239 (Abstr) |
| abstractText | Full text of Abstract: EXPRESSION OF TENASCIN IN THE MOUSE UTERUS DURING EARLY PREGNANCY. Kida H., Taga M., Minaguchi H., Hanazono M., Kusakabe M.*, Sakakura T.* Dept. of Obstet. and Gynecol., Yokohama City Univ. School of Medicine, Yokohama 236, Japan and The Institute of Physical and Chemical Research (RIKEN) Tsukuba Life Science Center*, Tsukuba 305, Japan. It is little known about a role of extracellular matrix (ECM) in the uterus in the early pregnancy. Tenascin (TN) is reported to be an ECM of glycoprotein expressing mainly in the tissues of developmental stage or carcinogenesis. To clarify the significance of TN in the uterus for implantation and maintenance of pregnancy, we examined the expression of TN in the mouse uterus during early pregnancy in both protein and molecular levels. The uteri of pregnant BALB/c mice of day 0, 1, 2, 3, 4, 5 (the day when vaginal plug was found is defined as day 0) were removed, fixed in 10% phosphate buffered formalin, and embedded in polyester wax. The localization of TN was observed immunohisto- chemically using anti-human TN antibody. After the extraction of total RNA from the uteri of the same stages of pregnant mice by single step method, Northern blot hybridization was performed using mouse TN cDNA probe. The localization of TN mRNA was also studied by in situ hybridization. In protein level, the expressions of TN were observed beneath the epithelium in day 0, 1, 2, 3, in the glandular basement membrane in day 0, 1, in muscular layer in day 1, 2, 3, 4, 5 , especially in day 4, 5, and in the implantation site in day 4, 5, respectively. The similar localization of TN mRNA was confirmed by in situ hybridization. Northern blot hybridization revealed that there were 2 kinds of band (7kb and 5.5kb) corresponding to TN mRNA in the pregnant mouse uterus of day 1, 2, 3 and that day 1 uterus had the strongest signal during early pregnancy. These results demonstrate that the expression of TN is corresponding to the tissue changes in the early pregnant uterus, suggesting that TN may play an important role in the uterus for maintaining the early pregnancy. |
