| First Author | Williamson CM | Year | 1997 |
| Journal | Genet Res | Volume | 70 |
| Pages | 87 | Mgi Jnum | J:44123 |
| Mgi Id | MGI:1099374 | Citation | Williamson CM, et al. (1997) Identification of a new imprinting region on distal mouse chromosome 2. Genet Res 70:87 |
| abstractText | Full text of Abstract: Identification of a new imprinting region on distal mouse chromosome 2. CHRISTINE M. WILLIAMSON1, NOBUAKI KIKYO2, COLIN V. BEECHEY1, SIMON T. BALL1, ROSALIND M. JOHN2, SHEILA C. BARTON2, FUMITOSHI ISHINO3, CHARLES TEASE1, ELIZABETH R. MARTIN1, BRUCE M. CATTANACH1, M. AZIM SURANI2 and JOSEPHINE PETERS1 1Mammalian Genetics Unit, MRC, Harwell, Didcot, Oxfordshire, OX11 ORD, UK; 2Wellcome/CRC Institute of Cancer and Developmental Biology, and Physiological Laboratory, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK; 3Gene Research Center, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midoriku, Yokohama 226, Japan. Distal chromosome (Chr) 2 is subject to parental imprinting, as mice carrying maternal duplication/ paternal deficiency for distal Chr 2 or the reciprocal genotype ? phenotypic abnormalities. A new imprinted gene, neuronatin (Nnat), has been found on distal Chr 2 using an mRNA differential display method. Nnat was shown to be paternally expressed in the central and peripheral nervous system and appeared to be a good candidate for the hypoactive phenotype associated with maternal duplication of the region. We looked at the expression of Nnat in duplication/deficient mice for distal Chr 2. Results showed that Nnat was not expressed in the brain of neonates carrying maternal duplication of the region distal to the T(2;8)26H breakpoint in band H1 but was expressed in the brain of neonates carrying maternal duplication of the region distal to the T(2;4)1Sn breakpoint, also in band 2H1. These results place Nnat between the translocation breakpoints of T26H and TlSn in band 2H1 and this mapping was verified by FISH and linkage analysis. Surprisingly, Nnat lies proximal to the reported minimal imprinting region located between 2H3 and 2H4, thus excluding Nnat as a candidate for the hypoactive phenotype and suggesting that there may be two distinct imprinting regions on distal Chr 2. |
