| First Author | Woodward EC | Year | 1993 |
| Journal | J Immunol | Volume | 150 |
| Issue | 8 | Pages | 43A (Abstr.) |
| Mgi Jnum | J:4568 | Mgi Id | MGI:53055 |
| Citation | Woodward EC, et al. (1993) Chromosomal mapping of the non-MHC linked proteasome genes. J Immunol 150(8):43A (Abstr.) |
| abstractText | Full text of Abstract: SATURDAY AM. MHC GENES AND GENETIC ORGANIZATION (230-233). 233. CHROMOSOMAL MAPPING OF THE NON-MHC LINKED PROTEASOME GENES. ELAINE C. WOODWARD AND JOHN J. MONACO. Department of Microbiology and Immunology, MCV Station Box 678, Virginia Commonwealth University, Richmond, VA 23298. The eucaryotic proteasome complex is made up of multiple polypeptide subunits which can be grouped into two distinct families whose members appear to have evolved through duplication and divergence of two ancestral proteasome genes. Tetrad analysis in Saccharomyces has shown that some of the yeast proteasome subunits are present as single copy genes and their expression is essential for cell survival. Identification of the chromosomal location for the mouse proteasome subunits is a first step in understanding the organization and regulation of these genes. Restriction fragment length polymorphisms (RFLP) and strain distribution patterns (SDP) for three sets of recombinant inbred mice have been used to determine the chromosomal location for the C3, C5, and C8 subunit genes using rat cDNA probes. The mouse C3 and C5 genes appear to be present as single copies on chromosome 13 and 18, respectively. RFLP and SDP analysis of the C8 subunit is consistent with the presence of at least three independently segregating copies of the C8 gene or related genes, with assignment of a C8 gene to mouse chromosome 3, 14, and 16. These results suggest that there are multiple copies of different proteasome genes in the mouse genome and to date none of the genes appear to be encoded as a gene complex on the same chromosome. (Supported by AI-33605) |
