| First Author | Baciu PC | Year | 1991 |
| Journal | J Cell Biol | Volume | 115 |
| Pages | 125a | Mgi Jnum | J:23985 |
| Mgi Id | MGI:71780 | Citation | Baciu PC, et al. (1991) Prediction of a novel integral membrane proteoglycan core protein related to Syndecan and Fibroglycan. J Cell Biol 115:125a |
| abstractText | Full text of Abstract: Tuesday. Glycosaminoglycans and Proteoglycans (725-730) 125a. 728. Prediction of a Novel Integral Membrane Proteoglycan Core Protein Related to Syndecan and Fibroglycan. P.C. Baciu, W.B. Howard and P.F. Goetinck. La Jolla Cancer Research Foundation, 10901 N. Torrey Pines Road, La Jolla, CA 92037. We have identified a cDNA clone (CE14B1) from a 10 day chicken embryo expression library with a polyclonal antiserum directed against a synthetic peptide whose sequence was based on the sequence of the cytoplasmic domain of murine syndecan (Saunders et al. JCB 108: 1547; 1989) The cDNA clone encodes an amino acid sequence that shares features of both syndecan and fibroglycan (Marynen et al., JBC 264: 7017; 1989). The amino acid sequence of both the cytoplasmic and transmembrane domains encoded by CE14B1 shows a high degree of similarity to the corresponding regions of syndecan and fibroglycan, whereas the degree of homology between the ectodomains is not as high. The amino acid sequence of the cytoplasmic domain has 72% and 82% homology with that of syndecan and fibroglycan respectively. This homology includes the four tyrosine residues characteristic of the cytoplasmic domains of both proteoglycans. The transmembrane domain predicted from CE14B1 has 54% and 75% homology with that of syndecan and fibroglycan respectively. The homology between the ectodomain deduced from CE14B1 with syndecan and fibroglycan is 41% and 38% respectively. Two serine-glycine sequences are present within the ectodomain predicted from CE14B1. These could represent sites for the glycanation of this putative proteoglycan core protein. The cDNA detects a 1.2 kB mRNA species in avian embryonic skin and in an avian hepatocyte cell line. The putative core protein encoded by the cDNA may represent a novel member of the syndecan family. (Supported by grants HD 22050, HD 25938 and T32 CA 09579) |
