| First Author | R??as E | Year | 2026 |
| Journal | STAR Protoc | Volume | 7 |
| Issue | 1 | Pages | 104376 |
| PubMed ID | 41701573 | Mgi Jnum | J:386463 |
| Mgi Id | MGI:8332179 | Doi | 10.1016/j.xpro.2026.104376 |
| Citation | Rias E, et al. (2026) Protocol for isolation of total RNA from mouse whole cochlea using organic solvents. STAR Protoc 7(1):104376 |
| abstractText | Determining gene expression changes in cochlear components is important for understanding the mechanisms underlying hearing loss. Here, we present a protocol for the isolation of total RNA from mouse cochleae across a wide range of ages (2-25 weeks old) using organic solvent extraction. We describe the steps for obtaining the entire cochlea from mice and isolating high-quality total RNA suitable for further reverse transcription and PCR. For complete details on the use and execution of this protocol, please refer to Rias et al.(1). |
