| First Author | Gibbs BC | Year | 2018 |
| Journal | Biochem Biophys Res Commun | Volume | 500 |
| Issue | 2 | Pages | 256-260 |
| PubMed ID | 29653101 | Mgi Jnum | J:271432 |
| Mgi Id | MGI:6280869 | Doi | 10.1016/j.bbrc.2018.04.054 |
| Citation | Gibbs BC, et al. (2018) beta1-integrin is a cell-autonomous factor mediating the Numb pathway for cardiac progenitor maintenance. Biochem Biophys Res Commun 500(2):256-260 |
| abstractText | Proper control of multipotent/stem cell number and fate is essential for ensuing organ formation during development. beta1-integrin, a subfamily of cell surface receptors, has a conserved role in maintenance of multipotent/stem cells, including renal progenitor cells, follicle stem cells, epidermal stem cells and neural stem cells. However, it remains unclear whether beta1-integrin has a role in cardiac progenitor cell (CPC) development. Here we show that a mesodermal deletion of beta1-integrin decreases Isl1(+) cell number in the second pharyngeal arch (PA2), where CPCs undergo renewal and expansion. Mesp1 lineage-specific mosaicism revealed that beta1-integrin-deleted Isl1(+) cells do not proliferate in the PA2. Consistently, beta1-integrin-deleted Isl1(+) CPCs failed to expand in vitro, independent of PA2 cells. beta1-integrin co-localized and physically associated with Numb, a crucial regulator of CPC renewal and expansion. Importantly, Numb/Numbl-deleted CPCs showed dramatic reduction in beta1-integrin levels. These findings suggest that beta1-integrin is a key mediator of the Numb pathway in CPC maintenance. |
