| First Author | Li J | Year | 2026 |
| Journal | Front Immunol | Volume | 17 |
| Mgi Jnum | J:393627 | Mgi Id | MGI:8461514 |
| Doi | 10.3389/fimmu.2026.1941021 | Citation | Li J, et al. (2026) An inducible human LDLR transgenic mouse model for enhanced lentiviral gene delivery to murine immune cells. Front Immunol 17 |
| abstractText | Lentivirus (LV)-based manipulation of immune cells is a commonly used approach in cell therapies. However, the most frequently used Vesicular Stomatitis Virus Glycoprotein (VSV-G) pseudotyping approach is inefficient to support LV gene delivery of mouse immune cells due to the lack of an optimal entry receptor. Here, we present a transgenic mouse model with Cre recombinase-inducible expression of the human Low Density Lipoprotein Receptor (hLDLR), the natural entry receptor of VSV-G LV. Surprisingly, we found that Cre induces robust hLDLR expression across multiple immune cells only under activation conditions. Induction of hLDLR expression by genetic crossing with Cre-transgenic mice or exposure to Cre mRNA-lipid nanoparticles (LNPs) potently sensitized murine immune cells, especially T cells, to VSV-G LV-mediated gene delivery. This novel transgenic mouse model serves as a valuable tool for investigating immune cell function and engineered cell therapies for cancer in syngeneic mouse models. |
