| First Author | Barson D | Year | 2020 |
| Journal | Nat Methods | Volume | 17 |
| Issue | 1 | Pages | 107-113 |
| PubMed ID | 31686040 | Mgi Jnum | J:371476 |
| Mgi Id | MGI:8238633 | Doi | 10.1038/s41592-019-0625-2 |
| Citation | Barson D, et al. (2020) Simultaneous mesoscopic and two-photon imaging of neuronal activity in cortical circuits. Nat Methods 17(1):107-113 |
| abstractText | Spontaneous and sensory-evoked activity propagates across varying spatial scales in the mammalian cortex, but technical challenges have limited conceptual links between the function of local neuronal circuits and brain-wide network dynamics. We present a method for simultaneous cellular-resolution two-photon calcium imaging of a local microcircuit and mesoscopic widefield calcium imaging of the entire cortical mantle in awake mice. Our multi-scale approach involves a microscope with an orthogonal axis design where the mesoscopic objective is oriented above the brain and the two-photon objective is oriented horizontally, with imaging performed through a microprism. We also introduce a viral transduction method for robust and widespread gene delivery in the mouse brain. These approaches allow us to identify the behavioral state-dependent functional connectivity of pyramidal neurons and vasoactive intestinal peptide-expressing interneurons with long-range cortical networks. Our imaging system provides a powerful strategy for investigating cortical architecture across a wide range of spatial scales. |
