| First Author | Pignatti E | Year | 2025 |
| Journal | Dev Biol | Volume | 528 |
| Pages | 204-215 | PubMed ID | 40915528 |
| Mgi Jnum | J:372665 | Mgi Id | MGI:8250005 |
| Doi | 10.1016/j.ydbio.2025.09.004 | Citation | Pignatti E, et al. (2025) CXXC Finger Protein 1 drives BMP signaling and progenitor cell differentiation during limb development. Dev Biol 528:204-215 |
| abstractText | The mechanisms mediating endochondral bone formation remain incompletely understood. Here, we show that CXXC Finger Protein 1 (CFP1) is required for the onset of chondrogenesis during forelimb development. CFP1-deficient mesenchymal progenitor cells (LMPs) retain an immature molecular signature with elevated FGF and SHH signaling and repressed BMP signaling, in part, due to (1) reduced expression of type I BMP receptors, (2) reduced Smad1 protein levels and (3) an altered extracellular niche. Moreover, the addition of exogenous BMP ligand or antagonism of heparan sulfate restores LMP differentiation toward a chondrogenic fate and enhances BMP signaling, suggesting a defect in BMP ligand bioavailability mediates the CFP1-deficient LMP phenotype. Together, these findings define CFP1 as a gatekeeper between the undifferentiated and differentiated state of LMPs during endochondral bone formation and as a physiological regulator of BMP signaling. CLASSIFICATION: Biological Sciences. |
