| First Author | Itkin T | Year | 2025 |
| Journal | Nat Immunol | Volume | 26 |
| Issue | 3 | Pages | 378-390 |
| PubMed ID | 40000903 | Mgi Jnum | J:389047 |
| Mgi Id | MGI:7868533 | Doi | 10.1038/s41590-025-02087-w |
| Citation | Itkin T, et al. (2025) Transcriptional activation of regenerative hematopoiesis via microenvironmental sensing. Nat Immunol 26(3):378-390 |
| abstractText | Transition between activation and quiescence states in hematopoietic stem and progenitor cells (HSPCs) is tightly governed by cell-intrinsic means and microenvironmental co-adaptation. Although this balance is fundamental for lifelong hematopoiesis and immunity, the underlying molecular mechanisms remain poorly defined. Multimodal analysis divulging differential transcriptional activity between distinct HSPC states indicates the presence of Fli-1 transcription factor binding motif in activated hematopoietic stem cells. We reveal that Fli-1 activity is essential during regenerative hematopoiesis in mice. Fli-1 directs activation programs while priming cellular sensory and output machineries, enabling HSPCs co-adoptability with a stimulated vascular niche through propagation of niche-derived angiocrine Notch1 signaling. Constitutively induced Notch1 signaling is sufficient to recuperate functional hematopoietic stem cells impairments in the absence of Fli-1, without leukemic transformation. Applying FLI-1 transient modified-mRNA transduction into latent adult human mobilized HSPCs, enables their niche-mediated expansion and superior engraftment capacities. Thus, decryption of stem cell activation programs offers valuable insights for immunological regenerative medicine. |
