| First Author | Effern M | Year | 2022 |
| Journal | STAR Protoc | Volume | 3 |
| Issue | 1 | Pages | 101038 |
| PubMed ID | 35059651 | Mgi Jnum | J:371089 |
| Mgi Id | MGI:8235925 | Doi | 10.1016/j.xpro.2021.101038 |
| Citation | Effern M, et al. (2022) CRISPitope: A generic platform to model target antigens for adoptive T cell transfer therapy in mouse tumor models. STAR Protoc 3(1):101038 |
| abstractText | This protocol details the procedure for CRISPR-assisted insertion of epitopes (CRISPitope), a flexible approach for generating tumor cells expressing model CD8(+) T cell epitopes fused to endogenously encoded gene products of choice. CRISPitope-engineered tumor cells can be recognized by T cell receptor-transgenic (TCRtg) CD8(+) T cells that are widely used in immunology research. Using mice inoculated with CRISPitope-engineered tumor cells, researchers can investigate how the choice of the target antigen for T cell immunotherapies influences treatment efficacy and resistance mechanisms. For complete details on the use and execution of this protocol, please refer to Effern et al. (2020). |
