Other
14 Authors
- Wang F,
- Rubenstein JL,
- Takatoh J,
- Ullian EM,
- Pai EL,
- Matcham AC,
- Yin C,
- Tsai NY,
- Wu K,
- Duan X,
- Yin L,
- Dang EJ,
- Toma K,
- Marciano DK
| First Author | Tsai NY | Year | 2022 |
| Journal | Nat Neurosci | Volume | 25 |
| Issue | 5 | Pages | 659-674 |
| PubMed ID | 35524141 | Mgi Jnum | J:367076 |
| Mgi Id | MGI:7482074 | Doi | 10.1038/s41593-022-01068-8 |
| Citation | Tsai NY, et al. (2022) Trans-Seq maps a selective mammalian retinotectal synapse instructed by Nephronectin. Nat Neurosci 25(5):659-674 |
| abstractText | The mouse visual system serves as an accessible model to understand mammalian circuit wiring. Despite rich knowledge in retinal circuits, the long-range connectivity map from distinct retinal ganglion cell (RGC) types to diverse brain neuron types remains unknown. In this study, we developed an integrated approach, called Trans-Seq, to map RGCs to superior collicular (SC) circuits. Trans-Seq combines a fluorescent anterograde trans-synaptic tracer, consisting of codon-optimized wheat germ agglutinin fused to mCherry, with single-cell RNA sequencing. We used Trans-Seq to classify SC neuron types innervated by genetically defined RGC types and predicted a neuronal pair from alphaRGCs to Nephronectin-positive wide-field neurons (NPWFs). We validated this connection using genetic labeling, electrophysiology and retrograde tracing. We then used transcriptomic data from Trans-Seq to identify Nephronectin as a determinant for selective synaptic choice from alphaRGC to NPWFs via binding to Integrin alpha8beta1. The Trans-Seq approach can be broadly applied for post-synaptic circuit discovery from genetically defined pre-synaptic neurons. |
