Other
14 Authors
- Berger DR,
- Jain V,
- Lynch S,
- Su Y,
- Carter JD,
- Fang T,
- Lichtman JW,
- Gupta S,
- Schalek RL,
- Janjic N,
- Lu X,
- Wu Y,
- Schneider DJ,
- Li PH
| First Author | Lu X | Year | 2026 |
| Journal | Nat Commun | Volume | 17 |
| Issue | 1 | PubMed ID | 42045230 |
| Mgi Jnum | J:388138 | Mgi Id | MGI:8355754 |
| Doi | 10.1038/s41467-026-72180-7 | Citation | Lu X, et al. (2026) Probing molecular diversity and ultrastructure of brain cells with fluorescent aptamers. Nat Commun |
| abstractText | Detergent-free immunolabeling has been proven feasible for volumetric correlated light and electron microscopy (vCLEM) with the application of small immunoprobes, providing a reliable method to superimpose essential molecular information of cells onto their high-resolution electron microscopic images. However, the application of this method is restricted by the availability of suitable affinity reagents. Here we introduce CAptVE, a method using slow off-rate modified aptamers for cell fluorescence labeling on ultrastructurally reconstructable electron micrographs. CAptVE provides labeling for a wide range of biomarkers, offering a pathway to integrate molecular analysis into volumetric electron microscopic studies of tissues, including neural circuit investigations via connectomics. |
