| First Author | G?mez HF | Year | 2023 |
| Journal | STAR Protoc | Volume | 4 |
| Issue | 2 | Pages | 102187 |
| PubMed ID | 36952332 | Mgi Jnum | J:380092 |
| Mgi Id | MGI:7640330 | Doi | 10.1016/j.xpro.2023.102187 |
| Citation | Gomez HF, et al. (2023) Time-lapse and cleared imaging of mouse embryonic lung explants to study three-dimensional cell morphology and topology dynamics. STAR Protoc 4(2):102187 |
| abstractText | Here, we present a protocol for collecting high-spatiotemporal-resolution datasets of undisturbed mouse embryonic epithelial rudiments using light-sheet fluorescence microscopy. We describe steps for rudiment dissection, clearing, and embedding for cleared and live imaging. We then detail procedures for light-sheet imaging followed by image processing and morphometric analysis. We provide protocol variations for imaging both growing and optically cleared lung explants to encourage the quantitative exploration of three-dimensional cell shapes, cell organization, and complex cell-cell dynamics. For complete details on the use and execution of this protocol, please refer to Gomez et al. (2021).(1). |
