| First Author | Crawford M | Year | 2023 |
| Journal | Bio Protoc | Volume | 13 |
| Issue | 17 | Pages | e4805 |
| PubMed ID | 37719067 | Mgi Jnum | J:380538 |
| Mgi Id | MGI:8279151 | Doi | 10.21769/BioProtoc.4805 |
| Citation | Crawford M, et al. (2023) Isolation, Purification, and Culture of Embryonic Melanoblasts from Green Fluorescent Protein-expressing Reporter Mice. Bio Protoc 13(17):e4805 |
| abstractText | In this article, we provide a method to isolate embryonic melanoblasts from reporter mouse strains. The mice from which these cells are isolated are bred into the ROSA26(mT/mG) reporter background, which results in green fluorescent protein (GFP) expression in the targeted melanoblast population. These cells are isolated and purified by fluorescence-activated cell sorting using GFP fluorescence. We also provide a method to culture the purified melanoblasts for further analysis. This method yields > 99% purity melanoblasts specifically targeted, and can be used for a variety of studies, including gene expression, clonogenic experiments, and biological assays, such as viability, capacity for directional migration, or differentiation into melanin-producing melanocytic cells. |
