| First Author | Hao Q | Year | 2024 |
| Journal | Int J Mol Sci | Volume | 25 |
| Issue | 8 | PubMed ID | 38673806 |
| Mgi Jnum | J:389040 | Mgi Id | MGI:7625270 |
| Doi | 10.3390/ijms25084220 | Citation | Hao Q, et al. (2024) Runx3 Regulates CD8(+) T Cell Local Expansion and CD43 Glycosylation in Mice by H1N1 Influenza A Virus Infection. Int J Mol Sci 25(8) |
| abstractText | We have recently reported that transcription factor Runx3 is required for pulmonary generation of CD8(+) cytotoxic T lymphocytes (CTLs) that play a crucial role in the clearance of influenza A virus (IAV). To understand the underlying mechanisms, we determined the effects of Runx3 knockout (KO) on CD8(+) T cell local expansion and phenotypes using an inducible general Runx3 KO mouse model. We found that in contrast to the lungs, Runx3 general KO promoted enlargement of lung-draining mediastinal lymph node (mLN) and enhanced CD8(+) and CD4(+) T cell expansion during H1N1 IAV infection. We further found that Runx3 deficiency greatly inhibited core 2 O-glycosylation of selectin ligand CD43 on activated CD8(+) T cells but minimally affected the cell surface expression of CD43, activation markers (CD44 and CD69) and cell adhesion molecules (CD11a and CD54). Runx3 KO had a minor effect on lung effector CD8(+) T cell death by IAV infection. Our findings indicate that Runx3 differently regulates CD8(+) T cell expansion in mLNs and lungs by H1N1 IAV infection. Runx3 is required for CD43 core 2 O-glycosylation on activated CD8(+) T cells, and the involved Runx3 signal pathway may mediate CD8(+) T cell phenotype for pulmonary generation of CTLs. |
