| First Author | Hong JP | Year | 2026 |
| Journal | STAR Protoc | Volume | 7 |
| Issue | 2 | Pages | 104586 |
| PubMed ID | 42172127 | Mgi Jnum | J:393025 |
| Mgi Id | MGI:8436727 | Doi | 10.1016/j.xpro.2026.104586 |
| Citation | Hong JP, et al. (2026) Protocol for an in vivo CRISPR screen for germinal center B cells in mice using ecotropic retrovirus. STAR Protoc 7(2):104586 |
| abstractText | The lack of an in vitro model that recapitulates germinal center (GC) B cell biology necessitates the use of animal models for genetic studies. Here, we present a protocol for an in vivo CRISPR screen for GC B cells in mice using an ecotropic retrovirus. We describe steps for constructing a single guide (sgRNA) library and performing a genetic screen in a mouse model of protein immunization, including procedures for sequencing and data analysis. |
