| First Author | Wang J | Year | 2022 |
| Journal | J Gastroenterol Hepatol | Volume | 37 |
| Issue | 4 | Pages | 734-740 |
| PubMed ID | 35102609 | Mgi Jnum | J:378270 |
| Mgi Id | MGI:8279128 | Doi | 10.1111/jgh.15790 |
| Citation | Wang J, et al. (2022) Nfatc1(+) colonic stem cells contribute to regeneration upon colitis. J Gastroenterol Hepatol 37(4):734-740 |
| abstractText | BACKGROUND AND AIM: Colonic stem cells play important roles in both normal epithelial turnover and injury repair. Lgr5(+) colonic stem cells are highly susceptible to DSS-induced damage. However, it is still unclear how colonic stem cells regenerate injured epithelium during colitis. Here, we explored the functions of a new population of NFATc1(+) colonic stem cells in experimental colitis. METHODS: Nfatc1(+) colonic stem cells were labeled using Nfatc1(CreERT2) ;R26(mTmG) reporter mice. Immunostaining assays were used to detect Goblet cells, enteroendocrine cells, and intestinal stem/progenitor cells. We performed lineage tracing assay to investigate whether Nfatc1(+) cells are real colonic stem cells using Nfatc1(CreERT2) ;R26(mTmG) mice. The contribution of Nfatc1(+) stem cells on epithelial regeneration was detected in experimental colitis induced by DSS. RESULTS: Nfatc1-reporter marked cells are enriched for +3 to +5 position in colonic crypts, and they are overlapped with Sox9(+) cells and Hopx(+) cells that have been identified as stem cells in small intestine. However, Nfatc1-reporter marked cells are not overlapped with Lgr5(+) colonic stem cells, as well as differentiated goblet cells and enteroendocrine cells. Furthermore, Nfatc1-reporter marked cells are able to give rise to all lineages of the colonic epithelium, and they preferentially contribute to the regeneration of colonic epithelium in DSS-induced experimental colitis. CONCLUSION: Nfatc1(+) cells were identified as a novel population of colonic stem cells that are primarily located at +3 to +5 position and contribute to epithelial regeneration during colitis. |
