| First Author | Luo PM | Year | 2025 |
| Journal | Angiogenesis | Volume | 28 |
| Issue | 4 | Pages | 57 |
| PubMed ID | 41099936 | Mgi Jnum | J:375526 |
| Mgi Id | MGI:8266350 | Doi | 10.1007/s10456-025-10000-0 |
| Citation | Luo PM, et al. (2025) Zonal endothelial cell heterogeneity underlies murine renal vascular development. Angiogenesis 28(4):57 |
| abstractText | The renal vasculature consists of highly specialized blood vessels with distinct physiological functions. Defining their transcriptional signatures and tracing their developmental ontogeny has thus far been challenging due to a lack of regionally specific endothelial biomarkers. Here, we performed single nuclear RNA sequencing (snucRNA-Seq) to interrogate the transcriptional heterogeneity of embryonic renal endothelial cells (ECs). We identified ten endothelial subtypes, and validated regionally restricted expression of novel marker genes of glomeruli, arteries, vasa recta, and immature capillary subtypes using multiplex RNAscope. We also define previously uncharacterized and heterogeneous molecular signatures of the immature renal vasculature, including putative endothelial progenitors. We interrogate biological characteristics of immature EC types using a variety of in vivo tools. Lineage tracing of Esm1-expressing cells reveals the previously unrecognized multi-origin and multi-clonal endothelial tip cell contribution to the glomerular vasculature. Together, this study provides a validated, tool-focused developmental atlas of the murine renal vasculature and elucidates novel cellular mechanisms of nephron vascularization. |
