| First Author | Hsu AY | Year | 2025 |
| Journal | STAR Protoc | Volume | 6 |
| Issue | 2 | Pages | 103886 |
| PubMed ID | 40503932 | Mgi Jnum | J:375514 |
| Mgi Id | MGI:8264874 | Doi | 10.1016/j.xpro.2025.103886 |
| Citation | Hsu AY, et al. (2025) Protocol to isolate and quantify large aging neutrophil-derived vesicles. STAR Protoc 6(2):103886 |
| abstractText | Large aging neutrophil-derived vesicles (LAND-Vs) are approximately 1 mum ectosomes released by aging neutrophils. Here, we present a protocol to identify and purify murine LAND-Vs from in vitro neutrophil cultures and ex vivo sources, including bone marrow, blood, and bronchoalveolar lavage fluid (BALF), using centrifugation and fluorescence-activated cell sorting (FACS). We also describe steps for isolation and quantification of human LAND-Vs from isolated neutrophils and human blood. For complete details on the use and execution of this protocol, please refer to Hsu et al.(1). |
