|  Help  |  About  |  Contact Us

Publication : A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex.

First Author  Yao Z Year  2021
Journal  Nature Volume  598
Issue  7879 Pages  103-110
PubMed ID  34616066 Mgi Jnum  J:382844
Mgi Id  MGI:7287830 Doi  10.1038/s41586-021-03500-8
Citation  Yao Z, et al. (2021) A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex. Nature 598(7879):103-110
abstractText  Single-cell transcriptomics can provide quantitative molecular signatures for large, unbiased samples of the diverse cell types in the brain(1-3). With the proliferation of multi-omics datasets, a major challenge is to validate and integrate results into a biological understanding of cell-type organization. Here we generated transcriptomes and epigenomes from more than 500,000 individual cells in the mouse primary motor cortex, a structure that has an evolutionarily conserved role in locomotion. We developed computational and statistical methods to integrate multimodal data and quantitatively validate cell-type reproducibility. The resulting reference atlas-containing over 56 neuronal cell types that are highly replicable across analysis methods, sequencing technologies and modalities-is a comprehensive molecular and genomic account of the diverse neuronal and non-neuronal cell types in the mouse primary motor cortex. The atlas includes a population of excitatory neurons that resemble pyramidal cells in layer 4 in other cortical regions(4). We further discovered thousands of concordant marker genes and gene regulatory elements for these cell types. Our results highlight the complex molecular regulation of cell types in the brain and will directly enable the design of reagents to target specific cell types in the mouse primary motor cortex for functional analysis.
Quick Links:
 
Quick Links:
 

Expression

Publication --> Expression annotations

 

Other

5 Bio Entities

0 Expression