| First Author | Deng ZM | Year | 1992 |
| Journal | Cytogenet Cell Genet | Volume | 60 |
| Pages | 170 (Abstr.) | Mgi Jnum | J:2040 |
| Mgi Id | MGI:50564 | Citation | Deng ZM, et al. (1992) Segments of homology between human 16p and mouse chromosomes. Cytogenet Cell Genet 60:170 (Abstr.) |
| abstractText | Full text of Abstract. 170 Human chromosome 16 workshop. Segments of Homology Between Human 16p and Mouse Chromosomes. Deng ZM1, Samson L2, Breuning M3, Siciliano MJ1; 1 Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston, TX, USA. 2 Molecular and Cellular Toxicology, Harvard School of Public Health, Boston, MA, USA. 3 Human Genetics, Leiden University, Leiden, The Netherlands. HGM10.5(1) indicates that the q-arm of human chromosome 16 appears to be extensively homologous with mouse chromosome 8. The proximal portion of the p-arm has a segment of homology with mouse chromosome 7 while a single point of homology has been identified between the HBA cluster at the end of 16p with the Hga locus on mouse chromosome 11. Identification of protamine 1 and 2 proximal to D16S60 and distal to D16S213 in the p13.13-p13.2 region of the chromosome 2 indicates another point of homology, this time with the centromeric region of mouse chromosome 16 where the homologues of the protamines have been identified. The single copy subclone (NOP3) of D16S237 was assigned proximal to the breakpoint defined by hybrid 23HA and distal to the one defined by hybrid CY196(2). This probe cross hybridized with rodent genomic DNA. On a mouse x CHO somatic cell hybrid panel informative for the segregation of mouse chromosomes both an 11 kb mouse XbaI and a 7.0 kb mouse HindIII fragment displayed 0% discordance with mouse chromosome 16 while discordancies for all other mouse chromosomes ranged from 29% - 59%. This result suggests a segment of homology from D16S237 - PRM1.2 in the human 16p13.13-p13.2 region and mouse chromosome 16. Genetic mapping in the mouse could confirm that segment should mouse Nop3 map in the centromeric region with the protamines. Identification of such a segment of shared homology has significant biomedical implications since that region of the mouse genome contains the SCID mutation and the human 16p13.13-p13.2 contains the ERCC4 repair gene as well as breakpoints associated with more than one human leukemia. Additionally, 3-alkyadenine DNA glycosylase (AAG) has recently been mapped to the end of the p-arm of human chromosome 16, 16p13.3(2). A mouse cDNA was isolated and also studied on the mouse-informative hybrid clone panel described above. Both mouse PstI and BamHI Aag fragments segregated concordantly with mouse chromosome 11, the site of mouse Hga(4). Therefore the HBA - AAG region at the end of human 16p suggests another new segment of homology between human and mouse chromosomes. 1 Davisson et al.: Cytogenet Cell Genet 55: 434-456 (1990) 2 Deng et al.: (This conference) 3 Reeves et al.: J Hered 1989, 442-446 (1989) 4 Lyon et al.: J Hered 1988, 93-95 (1988) |
