| First Author | Deng ZM | Year | 1992 |
| Journal | Am J Hum Genet | Volume | 51 |
| Issue | 4 | Pages | A394 (Abstr.) |
| Mgi Jnum | J:2927 | Mgi Id | MGI:51444 |
| Citation | Deng ZM, et al. (1992) Segments of homology between human 16p13 and mouse chromosomes. Am J Hum Genet 51(4):A394 (Abstr.) |
| abstractText | Full text of Abstract: A394 PUBLISHED ABSTRACTS. Physical Mapping (continued). Segments of homology between human 16p13 and mouse chromosomes. Z.M. Deng1, P. Liu1, K.R. Johnson2, M.T. Davisson2, M.H. Breuning3, L. Samson4, R.L. Stallings5, D.F. Callen6, M.J. Siciliano1. 1Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston, TX; 2The Jackson Laboratory, Bar Harbor, ME; 3Human Genetics, Leiden University. Leiden, The Netherlands; 4Molecular and Cellular Toxicology, Harvard School of Public Health, Boston, MA; 5Los Alamos National Laboratory, Los Alamos, NM; 6Cytogenetics and Molecular Genetics, Adelaide Children's Hospital, Adelaide, Australia. Two gene clusters (HBA complex and PRM1.2 from human chromosome 16p13 region have been shown to have homologs on mouse chromosome 11 and 16, respectively (HGM11). Here we report the refined localization of PRM1.2 and the mapping of additional markers which extended the homology between human 16pl3 and those mouse chromosomes. Both PRM1 and PRM2 were assigned to the same interval as D16S215 (proximal to D16S53 and distal to D16S53) confirming their p-arm assignments by in-situ hybridization and supporting their clustered configuration. A single copy subclone of D16S237, NOP3, was assigned proximal to the breakpoint defined by hybrid 23HA and distal to that of hybrid CY196 by Southern blot analysis of the broken 16 hybrid panel which consisted of 17 hybrids informative for different regions of human chromosome 16. NOP3 when hybridized to a mouse % CHO somatic cell hybrid panel informative for the segregation of mouse chromosomes showed 0% discordance with mouse chromosome 16 while the discordancies for all other mouse chromosomes ranged from 29% - 59%. This result suggests a segment of homology from D16S237 Ð PRM1.2 in the human 16p13.13- p13.2 region and mouse chromosome 16. Identification of such a segment of homology has significant biomedical implications since that region of mouse genome contains the SCID mutation and the human 16p13.13-13.2 contains the ERCC4 repair gene as well as breakpoints associated with more than one human leukemia. A cDNA from human 3-alkyladenine DNA glycosylase gene (AAG) was also hybridized to genomic DNAs from the broken 16 panel. The result put the AAG on 16p13.3 and was verified by fluorescent in situ hybridization. Southern hybridization of the AAG murine homologous gene to the mouse % CHO hybrid panel put both mouse PstI and BamHI Aag fragments on mouse chromosome 11, the site of mouse Hba. Therefore the HBA - AAG region represents a homologous segment between human 16p13.3 and mouse chromosome 11. Genetic length of the homologous segments on the mouse chromosome 11 and 16 will be presented. |
