| First Author | Barton DE | Year | 1987 |
| Journal | Cytogenet Cell Genet | Volume | 46 |
| Pages | 578 (Abstr.) | Mgi Jnum | J:4884 |
| Mgi Id | MGI:53365 | Citation | Barton DE, et al. (1987) INHA, INHBA, and INHBB, the loci for the three subunits of inhibin, mapped in mouse and man. Cytogenet Cell Genet 46:578 (Abstr.) |
| abstractText | Full text of Abstract: INHA, INHBA, and INHBB, the loci for the three subunits of inhibin, mapped in mouse and man. Barton DE,1 Yang-Feng TL,1 Mason AJ,2 Seeburg PH,2 Francke U.1 1Department of Human Genetics, Yale University School of Medicine, New Haven, CT. 2Department of Developmental Biology, Genentech, Inc, San Francisco, CA. Inhibin (INH) is a gonadal glycoprotein hormone which regulates pituitary FSH secretion. Inhibin exists in two forms, which share the same alpha subunit which, when covalently linked to one of two distinct Beta subunits (BetaA or BetaB), strongly inhibits pituitary FSH secretion. However, dimers of two Beta subunits (termed activin) are potent stimulators of FSH secretion and release, in vitro. These Beta subunits share extensive sequence homology with transforming growth factor Beta. Recently, cDNAs for all three inhibin subunits have been isolated (Mason, Niall and Seeburg. Biochem Biophys Res Commun 135:957, 1986). These cDNAs were used to map their cognate loci in both mouse and humans, by Southern blot analysis of somatic cell hybrid DNAs. The presence of a 9kb INHalpha hybridization signal was completely concordant with chromosome 2 in EcoRI-digests of DNA from 19 hybrid cell lines. When 12 hybrid cell lines were tested with the INHBetaA probe, complete concordance was observed with human chromosome 7. When HindIII digests of 15 hybrid cell DNAs were examined for the presence of a 15kb INHBetaB fragment, this gene was found to map to chromosome 2. Furthermore, analysis of four hybrids which contained rearrangements of chromosome 2 in the absence of the intact chromosome 2 allowed us to regionally localize INHA to 2q33-qter and INHBB to 2cen-ql3. In situ hybridization of 3P-labeled INHBetaA probe to metaphase chromosomes was used to obtain regional localization of this gene on HSA7. Of 106 grains seen over 77 cells, 19 (18.1%) were over region 7pl5-pl3, with a peak at 7p14. We therefore assign INHBA to chromosome 7, region p15-p13. In the mouse, a panel of 11 mouse x Chinese hamster hybrids and one mouse x rat hybrid was used to map the genes for INHalpha and INHBetaB (Inha and Inhbb) to chromosome 1, and for INHBetaA (Inhba) to mouse chromosome 13. Thus, INHA and INHBA join conserved linkage groups on HSA 2q32-qter/ MMU13 and HSA7/MMU13 respectively. HGM symbols: INHA, INHBA, INHBB. |
