| First Author | Szpirer C | Year | 1989 |
| Journal | Cytogenet Cell Genet | Volume | 51 |
| Pages | 1087 (Abstr.) | Mgi Jnum | J:12510 |
| Mgi Id | MGI:60755 | Citation | Szpirer C, et al. (1989) The rat gene map: assignment of 10 loci to Chromosome 5, and homology of rat Chromosome 5 with mouse Chromosome 4, human Chromosome 9 and human chromosome arm 1p. Cytogenet Cell Genet 51:1087 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of workshop presentations. The rat gene map: assignment of 10 loci to chromosome 5, and homology of rat chromosome 5 with mouse chromosome 4, human chromosome 9, and human chromosome arm 1p. (A2006) C Szpirer, M Riviere, J Szpirer, M Genet, P Dreze, G Levan Universite Libre de Bruxelles, Department of Molecular Biology, B1640 Rhode-St-Genese, Belgium; University of Gothenburg, Department of Genetics S400 33 Gothenburg, Sweden (LG) Using cloned probes in Southern blot analysis of DNA extracted from a panel of l4 independent mouse hepatoma x rat hepatocyte hybrids which segregate rat chromosomes, we determined the localization of the following 10 rat loci: alpha1-glycoprotein (orosomucoid; ORM), alpha2u-globulin (major urinary protein; MUP) atrial natriuretic factor (ANF = PND), glycoprotein-4-Beta-galactosyltransferase (GGTB), interferon alpha (IFNA, several genes), interferon Beta (IFNB), LCK and MOS oncogenes, D4Rp1 and a region of proviral insertion in MoMuLV-induced rat thymomas (DS1-1). All these loci cosegregated with rat chromosome 5 (at least 3 discordant clones were obtained for all the other chromosomes). Previously, we also assigned the MYCL oncogene and the aldolase B (ALDOB) gene to rat chromosome 5. These data show that rat chromosome 5 is highly homologous to mouse chromosome 4, known to carry most of these genes (Orm, Mup, Pnd, Ggtb, Ifa, Ifb, Lck, Mos, Dsi-1, D4Rp1, Mycl), and shares several loci with human chromosomes 9 (9q for ORM and 9p for the IFNA, IFNB and GGTB genes), or with the short arm of human chromosome 1 (1p carries PND, LCK and MYCL). In addition, using somatic cell hybrids possessing a single deleted rat chromosome 5, the rat IFN genes could be regionally localized to the region 5q22-23, which has also been shown to contain a locus suppressing the transformed phenotype (Islam et al., J. Cell Sci., in press); all the other loci maps outside region 5q22-23. On the other hand, we also found that the mouse Lck probe detects two non-syntenic genes in the rat genome: one maps on chromosome 5 (see above) and the other on chromosome 7 (which is partially homologous to mouse chromosome 15). We are grateful to those who kindly provided the molecular probes: H. Baumann (AGP), U. Schibler and F. Sierra (MUP), T. Inagami (ANF), N. Shaper (GGTB), T. Kos (IFNA), E. De Maeyer and Y. Kawade (Ifb), J. Marth (Lck), G. Vande Woude (Mos), D. Steffen (Dsi-1) and F. Berger (D4Rp1). |
