| First Author | Disteche CM | Year | 1989 |
| Journal | Cytogenet Cell Genet | Volume | 51 |
| Pages | 989-990 (Abstr.) | Mgi Jnum | J:12528 |
| Mgi Id | MGI:60773 | Citation | Disteche CM, et al. (1989) Regional localization of the Zfx gene in mouse and of the ZFX gene in human. Cytogenet Cell Genet 51:989-990 (Abstr.) |
| abstractText | Full text of Abstract: Regional localization of the Zfx gene in mouse and of the ZFX gene in human. (A2355). CM Disteche1, A de la Chapelle2, P Green3, DA Adler1, DC Page4. 1University of Washington, Seattle, WA; 2University of Helsinki, Helsinki, Finland; 3Department of Human Genetics Collaborative Research Inc., Bedford, MA; 4Whitehead Institute, Cambridge, MA. Regional localization of the Zfx gene in mouse was achieved by in situ hybridization to mouse metaphase chromosomes. Probe pDP1068, a 2.1-kb genomic EcoRI fragment, was labeled with 3H. Chromosomes were identified with Q-banding. A significant accumulation of grains was found on three different mouse chromosomes. Of 76 sites of hybridization, 15 (20%) were located on the X chromosome, 5 (7%) on the Y chromosome, and 26 (36%) on chromosome 10. The peaks of hybridization were located at bands D and C of the X chromosome, at band A1 and on the short arm of the Y chromosome and at bands B3 and B4 of chromosome 10, respectively. Hybridization to the mouse X chromosome sublocalizes Zfx to band D or C. The weak hybridization signal on the mouse Y chromosome is due to cross hybridization of the probe to the Zfy genes. Hybridization to mouse chromosome 10 sublocalizes a related gene or a pseudogene of Zfx to band B3 or B4. In situ hybridization to human metaphase chromosomes was carried out using probe pDP1039, a 1.2-kb genomic HindIII fragment labeled with 3H. Chromosomes were identified with G-banding. A single hybridization site was found on human chromosomes using a probe for ZFX. Twenty percent of a total of 196 autographic grains examined were located on the short arm of the human X chromosome with a peak at bands Xp21.3-p22.1. Meiotic linkage mapping of ZFX was done using the same probe, pDP 1039, which detects an RFLP with Msp1 digestion of DNA. Multipoint analysis resulted in a linkage map placing ZFX about 16 cM distal to DXS164 which is part of the DMD gene (in Xp21.2) and 9 cM poximal to DXS41 located in Xp22.1. Comparison of the data in mouse and in human indicates that the ZFX gene lies in a conserved region of the human and mouse X chromosomes that includes the Duchenne muscular dystrophy locus. |
