| First Author | Zuberi AR | Year | 1996 |
| Journal | Mouse Genome | Volume | 94 |
| Issue | 1 | Pages | 152-4 |
| Mgi Jnum | J:40329 | Mgi Id | MGI:87670 |
| Citation | Zuberi AR, et al. (1996) Allele sizes at Chromosome 2 Mit loci from 129/J, 129/Ola, C57BL/10J, and the autoimmune-prone mouse strains BXSB/MpJ-Yaa, MRL/MpJ, SJL/J and NZB/B1NJ. Mouse Genome 94(1):152-4 |
| abstractText | Full text of Mouse Genome contribution: ALLELE SIZES AT CHROMOSOME 2 Mit LOCI FROM 129/J, 129/Ola, C57BL/10J, AND THE AUTOIMMUNE-PRONE MOUSE STRAINS BXSB/MpJ-Yaa, MRL/MpJ, SJL/J AND NZB/B1NJ. Aamir R. Zuberi, Gregory J. Christianson and Derry C. Roopenian; The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609. Introduction Genetic linkage analysis in the mouse has been greatly simplified by the availability of a large number of locus specific PCR primers that amplify simple sequence (CA)n repeats (SSR's). These sequences are often length polymorphic between different inbred mouse strains and multiple alleles sometimes exist at any specified locus. These genetic markers, including the allele sizes for these SSRs have been reported for a panel of 12 inbred strains(1-3). Other inbred mouse strains exist that possess unique immunological, behavioral or physiological characteristics, and it would be useful if the allele sizes for these other strains were known so that subsequent genetic manipulations would be easier to perform. We are interested in determining the role that Beta2-microglobulin plays in disease progression in several established mouse models of autoimmunity. Congenic lines were generated from selected autoimmune strains that were homozygous for a targeted B2mtm1Unc null mutation on Chromosome 2. This mutation was generated by replacement of B2m in 129/Ola-derived embryonic stem cells with an inactive gene by homologous recombination4, 5. The immunological characterization of these strains will be reported elsewhere (in preparation). We report here the allele sizes of selected D2Mit loci in seven previously untyped inbred strains. These include the autoimmune prone strains, BXSB/MpJ-Yaa, MRL/MpJ, SJL/J and NZB/B1NJ, two 129 substrains, 129/J and 129/Ola and C57BL/10J. Materials and Methods A breeding colony of B2m - deficient mice was obtained from B. Koller and O. Smithies (University of North Carolina, Chapel Hill, NC). Other mice used were obtained from the animal resources of The Jackson Laboratory. Congenic lines were established at N11. Genomic DNA was prepared from mouse peripheral blood lymphocytes. A selection of PCR primers (Research Genetics Inc., AL) were chosen that mapped to different loci along the length of Chromosome 2. PCR products were labeled by alpha32P.dcTP incorporation during DNA amplification, or by direct labeling of one of the PCR primers with gamma-32 P ATP. Standard PCR amplification conditions were used1. Reaction products were resolved on 4 - 6% denaturing polyacrylamide gels and visualized after auto- radiography. Allele sizes of previously untyped strains were determined by comigration with one of the alleles of a control strain for which the sizes have been reported3. These control mice included BALB/cJ, C57BL/6J, C3H/HeJ, NOD/Lt and AKR/J. In a few cases the 129/J alleles were only scored relative to C57BL/10J and an allele size was not determined. Results and Discussion Table 1 lists the observed allele sizes for the seven inbred strains. For the markers examined, we observed no difference in allele sizes between the 129/Ola substrain, the source of the donor DNA segment in the congenic lines, and 129/J. There were also no differences in the allele sizes between C57BL/10J and C57BL/6J. These data should be beneficial in the genetic manipulation of the strains examined and the characterization of Recombinant Inbred (Rl) stocks derived from these progenitor strains. The information should also facilitate the genetic manipulation of stocks carrying targeted mutations on Chromosome 2, when the mutations are generated in 129/Ola or 129/J-derived embryonic stem cells. Acknowledgments We thank Drs. Beverly Koller and Oliver Smithies for B2mtm1Unc targeted mice. The technical assistance of Theresa Hopkins is gratefully acknowledged. The critical reviews of the manuscript by Drs. Verity Letts and Elizabeth M. Simpson is also appreciated. This work was supported by National Institutes of Health grants R01-AI28802, R01-AI24544 to D.C.R. and by Core grant CA34196 to the Shared services facilities of the Jackson Laboratory. REFERENCES 1. Dietrich, W. et al. (1992). Genetics 131: 423-447. 2. Dietrich, W. F. et al. (1994). Nat. Genet. 7: 220-245. 3. The Whitehead Institute/MIT Center for Genome Research, Genetic Map of the Mouse, Database Release 10, April 28, 1995 (URL: http://www-genome.wi.mit.edu) 4. Koller, B.H., Smithies, O. (1989). Proc. Natl. Acad. Sci. USA 86: 8932-8935. 5. Koller, B.H. et al. (1990). Science 248: 1227-1230. Table 1: Allele sizes (bp) of selected Chromosome 2 SSR markers Locus: D2Mit1; cM from centromere: 2.4; C57BL/6J, C57BL/10J: 124; 129/J: >B10c. Locus: D2Mit3; cM from centromere: 8.0; C57BL/6J, C57BL/10J: 160; 129/J: 160. Locus: D2Mit7; cM from centromere: 30.6; C57BL/6J, C57BL/10J: 147; 129/J: <B10c. Locus: D2Mit9; cM from centromere: 41.8; C57BL/6J, C57BL/10J: 190; 129/J: 182; BXSB-Yaa: 182; MRL/MpJ: 190; SJL/J: 185; NZB/B1NJ: 190. Locus: D2Mit11; cM from centromere: 46.3; C57BL/6J, C57BL/10J: 226; 129/J: 226; BXSB-Yaa: 232; MRL/MpJ: 232; SJL/J: 226; NZB/B1NJ: 226. Locus: D2Mit13; cM from centromere: 54.1; C57BL/6J, C57BL/10J: 190; 129/J: 193; 129/Ola: 193; BXSB-Yaa: 190; MRL/MpJ: 192; SJL/J: 193; NZB/B1NJ: 193. Locus: D2Mit14; cM from centromere: 53.0; C57BL/6J, C57BL/10J: 142; 129/J: 142; 129/Ola: 142; BXSB-Yaa: 130; MRL/MpJ: 130; SJL/J: 125; NZB/B1NJ: 130. Locus: D2Mit16; cM from centromere: 60.8; C57BL/6J, C57BL/10J: 238; 129/J: >B10c. Locus: D2Mit17; cM from centromere: 60.8; C57BL/6J, C57BL/10J: 205; 129/J: 214. Locus: D2Mit21; cM from centromere: 69.7; C57BL/6J, C57BL/10J: 258; 129/J: 258. Locus: D2Mit26; cM from centromere: 75.3; C57BL/6J, C57BL/10J: 195; 129/J: 210; 129/Ola: 210; BXSB-Yaa: 210; MRL/MpJ: 195; SJL/J: 195; NZB/B1NJ: 197. Locus: D2Mit30; Gene: Tmah; cM from centromere: 60.8; C57BL/6J, C57BL/10J: 320; 129/J: <B10c. Locus: D2Mit46; cM from centromere: 65.2; C57BL/6J, C57BL/10J: 118; 129/J: 116; 129/Ola: 116; BXSB-Yaa: 118; MRL/MpJ: 116; SJL/J: 116; NZB/B1NJ: 118. Locus: D2Mit50; cM from centromere: 83.6; C57BL/6J, C57BL/10J: 152; 129/J: 154. Locus: D2Mit51; cM from centromere: 83.6; C57BL/6J, C57BL/10J: 128; 129/J: <B10c. Locus: D2Mit62; Gene: Thbs1; cM from centromere: 58.6; C57BL/6J, C57BL/10J: 162; 129/J: 146; 129/Ola: 146; BXSB-Yaa: 162; MRL/MpJ: 162; SJL/J: 146; NZB/B1NJ: 146. Locus: D2Mit63; cM from centromere: 58.6; C57BL/6J, C57BL/10J: 212; 129/J: 218; 129/Ola: 218; BXSB-Yaa: 212; MRL/MpJ: 212; SJL/J: 232; NZB/B1NJ: 218. Locus: D2Mit104; cM from centromere: 59.7; C57BL/6J, C57BL/10J: 148; 129/J: <B10c. Locus: D2Mit108; cM from centromere: 67.5; C57BL/6J, C57BL/10J: 192; 129/J: 192; 129/Ola: 192; BXSB-Yaa: 192; MRL/MpJ: 192; SJL/J: 192; NZB/B1NJ: 192. Locus: D2Mit109; cM from centromere: 70.8; C57BL/6J, C57BL/10J: 176; 129/J: 164; 129/Ola: 164; BXSB-Yaa: 164; MRL/MpJ: 176; SJL/J: 176; NZB/B1NJ: 164. Locus: D2Mit133; cM from centromere: 59.7; C57BL/6J, C57BL/10J: 203; 129/J: 207. Locus: D2Mit134; Gene: Ckmt1; cM from centromere: 60.8; C57BL/6J, C57BL/10J: 200; 129/J: 196. Locus: D2Mit190; cM from centromere: 60.8; C57BL/6J, C57BL/10J: 123; 129/J: 149. Locus: D2Mit223; cM from centromere: 65.2; C57BL/6J, C57BL/10J: 99; 129/J: 99; 129/Ola: 99; BXSB-Yaa: 99; MRL/MpJ: 143; SJL/J: 143; NZB/B1NJ: 99. Locus: D2Mit254; cM from centromere: 57.5; C57BL/6J, C57BL/10J: 199; 129/J: 213; 129/Ola: 213; BXSB-Yaa: 199; MRL/MpJ: 208; SJL/J: 211; NZB/B1NJ: 206. Locus: D2Mit255; cM from centromere: 60.8; C57BL/6J, C57BL/10J: 153; 129/J: 160. Locus: D2Mit258; cM from centromere: 66.4; C57BL/6J, C57BL/10J: 108b; 129/J: 118; 129/Ola: 118; BXSB-Yaa: 118; MRL/MpJ: 108; SJL/J: 118; NZB/B1NJ: 118. Locus: D2Mit276; cM from centromere: 58.6; C57BL/6J, C57BL/10J: 144; 129/J: 140; 129/Ola: 140; BXSB-Yaa: 144; MRL/MpJ: 144; SJL/J: 140; NZB/B1NJ: 152. Locus: D2Mit277; cM from centromere: 60.8; C57BL/6J, C57BL/10J: 274b; 129/J: 303. Locus: D2Mit280; cM from centromere: 70.8; C57BL/6J, C57BL/10J: 200; 129/J: 200; 129/Ola: 200; BXSB-Yaa: 200; MRL/MpJ: 192; SJL/J: 192; NZB/B1NJ: 200. Locus: D2Mit304; cM from centromere: 63.0; C57BL/6J, C57BL/10J: 120; 129/J: 136; 129/Ola: 136; BXSB-Yaa: 120; MRL/MpJ: 120; SJL/J: 136; NZB/B1NJ: 130. Locus: D2Mit395; cM from centromere: 59.7; C57BL/6J, C57BL/10J: 128; 129/J: 162. Locus: D2Mit396; cM from centromere: 60.8; C57BL/6J, C57BL/10J: 120; 129/J: 99. Locus: D2Mit397; cM from centromere: 60.8; C57BL/6J, C57BL/10J: 149; 129/J: 131. Locus: D2Mit445; cM from centromere: 60.8; C57BL/6J, C57BL/10J: 121; 129/J: 87. Locus: D2Mit446; cM from centromere: 60.8; C57BL/6J, C57BL/10J: 124; 129/J: 122. Locus: D2Mit484; cM from centromere: 59.7; C57BL/6J, C57BL/10J: 112; 129/J: 132. Locus: D2Nds1; cM from centromere: 54.1; C57BL/6J, C57BL/10J: 174; 129/J: 178. Locus: D2Nds3; Gene: Il1b; cM from centromere: 65.2; C57BL/6J, C57BL/10J: 280; 129/J: 270; 129/Ola: 270; BXSB-Yaa: 280; MRL/MpJ: 280; SJL/J: 270; NZB/B1NJ: 270. (a) From reference 3. (b) Allele size is different from that listed in reference 3. (c) Locus only typed by relative mobility |
