| First Author | Yum HW | Year | 2018 |
| Journal | Biochem Biophys Res Commun | Volume | 502 |
| Issue | 1 | Pages | 1-8 |
| PubMed ID | 29775616 | Mgi Jnum | J:273424 |
| Mgi Id | MGI:6276792 | Doi | 10.1016/j.bbrc.2018.05.093 |
| Citation | Yum HW, et al. (2018) Amelioration of UVB-induced oxidative stress and inflammation in fat-1 transgenic mouse skin. Biochem Biophys Res Commun 502(1):1-8 |
| abstractText | omega-3 polyunsaturated fatty acids (PUFAs), which are abundant in fish oils, are known to scavenge lipid peroxyl radicals and potentiate host immune defence. As UVB-induced oxidative stress and inflammation have been implicated in apoptotic cell death, this study was aimed to investigate the anti-inflammatory, anti-oxidative, and anti-apoptotic capacity of fat-1 transgenic mice capable of converting omega-6 to omega-3 PUFAs. Wild-type (WT) C57BL/6 mice and fat-1 mice were maintained on the AIN-93 diet supplemented with 10% safflower oil rich in omega-6 PUFAs for 5 weeks. The omega-3/omega-6 PUFA ratio was significantly higher in the dorsal skin of fat-1 mice than that in the WT mice. Upon single exposure to UVB (5.0kJ/m(2)) radiation, fat-1 mice showed inflammatory as well as oxidative tissue damage and the expression of pro-inflammatory enzymes, cyclooxygenases-2 and inducible nitric oxide synthase in the skin to a much lesser extent than the WT mice. The protection of fat-1 mice from UVB-induced skin inflammation was associated with decreased phosphorylation of STAT3. Moreover, UVB-induced apoptosis was attenuated in fat-1 mouse skin. In comparison to WT animals, higher levels of Nrf2 and its target proteins, such as heme oxygenase-1, NAD(P)H:quinone oxidoreductase-1 and thioredoxin-1, were found in the skin of fat-1 mice. |
