| First Author | Meng X | Year | 2019 |
| Journal | J Neurophysiol | Volume | 122 |
| Issue | 5 | Pages | 1962-1974 |
| PubMed ID | 31533018 | Mgi Jnum | J:365739 |
| Mgi Id | MGI:8188234 | Doi | 10.1152/jn.00828.2018 |
| Citation | Meng X, et al. (2019) Increasing the expression level of ChR2 enhances the optogenetic excitability of cochlear neurons. J Neurophysiol 122(5):1962-1974 |
| abstractText | Optogenetics comprise a promising alternative to electrical stimulation for characterization of neural circuits and for the next generation of neural prostheses. Optogenetic stimulation relies on expression of photosensitive microbial proteins in animal cells to initiate a flow of ions into the cells in response to visible light. Here, we generated a novel transgenic mouse model in which we studied the optogenetic activation of spiral ganglion neurons, the primary afferent neurons of the auditory system, and showed a strong optogenetic response, with a similar amplitude as the acoustically evoked response. A twofold increase in the level of channelrhodopsin expression significantly increased the photosensitivity at both the single cell and organismal levels but also partially compromised the native electrophysiological properties of the neurons. The importance of channelrhodopsin expression level to optogenetic stimulation, revealed by these quantitative measurements, will be significant for the characterization of neural circuitry and for the use of optogenetics in neural prostheses.NEW & NOTEWORTHY This study reveals a dose-response relationship between channelrhodopsin expression and optogenetic excitation. Both single cell and organismal responses depend on the expression level of the heterologous protein. Expression level of the opsin is thus an important variable in determining the outcome of an optogenetic experiment. These results are key to the implementation of neural prostheses based on optogenetics, such as next generation cochlear implants, which would use light to elicit a neural response to sound. |
