| First Author | Ganji E | Year | 2023 |
| Journal | Sci Adv | Volume | 9 |
| Issue | 25 | Pages | eadf4683 |
| PubMed ID | 37352350 | Mgi Jnum | J:362999 |
| Mgi Id | MGI:7495207 | Doi | 10.1126/sciadv.adf4683 |
| Citation | Ganji E, et al. (2023) Optogenetic-induced muscle loading leads to mechanical adaptation of the Achilles tendon enthesis in mice. Sci Adv 9(25):eadf4683 |
| abstractText | Skeletal shape depends on the transmission of contractile muscle forces from tendon to bone across the enthesis. Loss of muscle loading impairs enthesis development, yet little is known if and how the postnatal enthesis adapts to increased loading. Here, we studied adaptations in enthesis structure and function in response to increased loading, using optogenetically induced muscle contraction in young (i.e., growth) and adult (i.e., mature) mice. Daily bouts of unilateral optogenetic loading in young mice led to radial calcaneal expansion and warping. This also led to a weaker enthesis with increased collagen damage in young tendon and enthisis, with little change in adult mice. We then used RNA sequencing to identify the pathways associated with increased mechanical loading during growth. In tendon, we found enrichment of glycolysis, focal adhesion, and cell-matrix interactions. In bone, we found enrichment of inflammation and cell cycle. Together, we demonstrate the utility of optogenetic-induced muscle contraction to elicit in vivo adaptation of the enthesis. |
