| First Author | Zheng L | Year | 2008 |
| Journal | Transgenic Res | Volume | 17 |
| Issue | 4 | Pages | 741-7 |
| PubMed ID | 18183494 | Mgi Jnum | J:168455 |
| Mgi Id | MGI:4888309 | Doi | 10.1007/s11248-007-9160-5 |
| Citation | Zheng L, et al. (2008) A one-plasmid conditional color-switching transgenic system for multimodal bioimaging. Transgenic Res 17(4):741-7 |
| abstractText | We have developed a new construct to generate transgenic mice with one plasmid that offers: (1) Cre/loxP-mediated spatial and temporally-controlled tissue-specific transgene expression; (2) A color-switching mechanism that uses spectrum-complementary genetically-encoded red (mRFP) and green (eGFP) fluorescent markers to label the transgene-expressing cells; (3) A bioluminescent marker that turns-on in the transgene-expressing cells; (4) eGFP as a cell surface marker in the transgene-expressing cells that facilitates the isolation and targeting of these cells. This vector was tested in vitro by co-transfection of the transgenic plasmid and a plasmid containing Cre recombinase into cultured cells and by establishing a transgenic mouse line. We show that this method allows versatile transgene expression targeting and color-switching to facilitate fluorescent and bioluminescent imaging both in cultured cells and in vivo. Our strategy provides time-saving features in tissue-specific transgene expression, bioimaging and primary cell isolation and can be used for generation of gene-specific transgenic mice. |
