| Primary Identifier | MGI:5314229 | Allele Type | Transgenic |
| Attribute String | Inducible, Recombinase, Reporter | Gene | Tg(Krt8-cre/ERT2,-GFP)1Xin |
| Strain of Origin | C57BL/6J | Induced With | tamoxifen |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | A BAC clone (RP23-234D2) was modified by recombineering methods to insert the cre/ERT2 fusion sequence at the translational start codon of Krt8. An IRES-GFP expression cassette was inserted downstream of the cre sequence. The linearized BAC construct was microinjected into fertilized C57BL/6J oocytes. RT-PCR was used to screen founder mice and one line was chosen for characterization based on its cre/ERT2 expression pattern which closely recapitulated endogenous Krt8 expression. This line carries 2 copies of the transgene (based on quantitative PCR performed on genomic DNA) with a single insertion site. However, although GFP is detectable by RT-PCR and Western blot analysis, GFP fluorescence cannot be visualized by fluorescent microscopy in tissue sections or by FACS analysis of dissociated prostate cells. |
