| Primary Identifier | MGI:5550468 | Allele Type | Transgenic |
| Attribute String | Recombinase | Gene | Tg(Ucp1-cre)1Evdr |
| Strain of Origin | FVB | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | The C57BL/6 mouse bacterial artificial chromosome (BAC) 148M1 containing the entire Ucp1 gene, and other genes, was modified by inserting sequence encoding cre recombinase, into the translation initiation site into exon 1 of the Ucp1 gene. An FRT site flanked KAN cassette and a loxP site were removed. DNA from the modified BAC clone was used as the source for the transgene, which was microinjected into the pronucleus of FVB fertilized eggs. A founder line was subsequently established. The insertion site was localized to mouse chromosome 1 and caused a deletion from Chr1:20,962,125 to Chr1:21,016,858 and a 3' approximately 280 kb inversion of genomic sequences. This directly deletes or inverts all or large parts of the Efhc1, Tram2, Tmem14a, and Gsta3 genes. |
