| Primary Identifier | MGI:8437132 | Allele Type | Endonuclease-mediated |
| Gene | Cdh5 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Aspartic acid codon 646 (GAT) and glutamic acid codons 647 and 648 (GAG) were changed top alanine (GCA) (p.D646_E648delinsAAA) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation disrupts binding of the encoded protein to the Ctnnd1 protein, which leads to microvascular patterning defects. |
